FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1631475273> ?p ?o ?g. }

• W1631475273 endingPage "19952" @default.
• W1631475273 startingPage "19945" @default.
• W1631475273 abstract "Chromatin high mobility group protein I (HMG-I) is a mammalian nonhistone protein that has been demonstrated both in vitro and in vivo to preferentially bind to A.T-rich sequences of DNA. Recently DNA-binding domain peptide that specifically mediates in vitro interaction of high mobility group protein (HMG)-I with narrow minor groove of A.T-DNA has been experimentally determined. Because of its predicted secondary structure, binding domain peptide has been called the A.T motif. Previously we demonstrated that A.T hook of murine HMG-I protein is specifically phosphorylated by purified mammalian cdc2 kinase in vitro and that same site(s) are also phosphorylated in vivo in metaphase-arrested cells. We also found that DNA binding affinity of short synthetic binding domain peptides phosphorylated in vitro by cdc2 kinase was significantly reduced compared with unphosphorylated peptides. Here we extend these findings to intact natural and recombinant HMG-I proteins. We report that affinity of binding of full-length HMG-I proteins to A.T-rich sequences is highly dependent on ionic conditions and that phosphorylation of intact proteins by cdc2 kinase reduces their affinity of in vitro binding to A.T-DNA by about 20-fold when assayed near normal mammalian physiological salt concentrations. Furthermore, in cell synchronization studies, we demonstrated that murine HMG-I proteins are phosphorylated in vivo in a cell cycle-dependent manner on same amino acid residues modified by purified cdc2 kinase in vitro. Together these results strongly support assertion that HMG-I proteins are natural substrates for mammalian cdc2 kinase in vivo and that their cell cycle-dependent phosphorylation by this enzyme(s) significantly modulates their DNA binding affinity, thereby possibly altering their biological function(s)." @default.
• W1631475273 created "2016-06-24" @default.
• W1631475273 creator A5048318823 @default.
• W1631475273 creator A5070796998 @default.
• W1631475273 creator A5083361673 @default.
• W1631475273 date "1991-10-01" @default.
• W1631475273 modified "2023-10-18" @default.
• W1631475273 title "Phosphorylation by cdc2 kinase modulates DNA binding activity of high mobility group I nonhistone chromatin protein." @default.
• W1631475273 cites W1519139333 @default.
• W1631475273 cites W1541214688 @default.
• W1631475273 cites W1547579138 @default.
• W1631475273 cites W1548068969 @default.
• W1631475273 cites W1550029130 @default.
• W1631475273 cites W1553673115 @default.
• W1631475273 cites W1569365736 @default.
• W1631475273 cites W1669933714 @default.
• W1631475273 cites W1782902010 @default.
• W1631475273 cites W1873199501 @default.
• W1631475273 cites W1963590275 @default.
• W1631475273 cites W1966797426 @default.
• W1631475273 cites W1977496565 @default.
• W1631475273 cites W1977646528 @default.
• W1631475273 cites W1980585800 @default.
• W1631475273 cites W1983751139 @default.
• W1631475273 cites W1985527733 @default.
• W1631475273 cites W1985834906 @default.
• W1631475273 cites W1991917303 @default.
• W1631475273 cites W1995527354 @default.
• W1631475273 cites W1996991816 @default.
• W1631475273 cites W1998313627 @default.
• W1631475273 cites W1999922219 @default.
• W1631475273 cites W2000146554 @default.
• W1631475273 cites W2007473965 @default.
• W1631475273 cites W2019070778 @default.
• W1631475273 cites W2023427346 @default.
• W1631475273 cites W2024470778 @default.
• W1631475273 cites W2026884660 @default.
• W1631475273 cites W2027305883 @default.
• W1631475273 cites W2030019692 @default.
• W1631475273 cites W2033777731 @default.
• W1631475273 cites W2042715516 @default.
• W1631475273 cites W2044858791 @default.
• W1631475273 cites W2047295125 @default.
• W1631475273 cites W2047799300 @default.
• W1631475273 cites W2055119614 @default.
• W1631475273 cites W2058126868 @default.
• W1631475273 cites W2059722081 @default.
• W1631475273 cites W2060633601 @default.
• W1631475273 cites W2062579188 @default.
• W1631475273 cites W2068034418 @default.
• W1631475273 cites W2072722583 @default.
• W1631475273 cites W2081682538 @default.
• W1631475273 cites W2082139304 @default.
• W1631475273 cites W2083024364 @default.
• W1631475273 cites W2083953218 @default.
• W1631475273 cites W2088134192 @default.
• W1631475273 cites W2088514626 @default.
• W1631475273 cites W2092383763 @default.
• W1631475273 cites W2093915334 @default.
• W1631475273 cites W2102242413 @default.
• W1631475273 cites W2115659567 @default.
• W1631475273 cites W2117939247 @default.
• W1631475273 cites W2123930515 @default.
• W1631475273 cites W34532530 @default.
• W1631475273 cites W45295259 @default.
• W1631475273 cites W86385217 @default.
• W1631475273 doi "https://doi.org/10.1016/s0021-9258(18)54874-2" @default.
• W1631475273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1939057" @default.
• W1631475273 hasPublicationYear "1991" @default.
• W1631475273 type Work @default.
• W1631475273 sameAs 1631475273 @default.
• W1631475273 citedByCount "122" @default.
• W1631475273 countsByYear W16314752732012 @default.
• W1631475273 countsByYear W16314752732014 @default.
• W1631475273 countsByYear W16314752732015 @default.
• W1631475273 countsByYear W16314752732016 @default.
• W1631475273 countsByYear W16314752732018 @default.
• W1631475273 countsByYear W16314752732019 @default.
• W1631475273 countsByYear W16314752732020 @default.
• W1631475273 countsByYear W16314752732021 @default.
• W1631475273 crossrefType "journal-article" @default.
• W1631475273 hasAuthorship W1631475273A5048318823 @default.
• W1631475273 hasAuthorship W1631475273A5070796998 @default.
• W1631475273 hasAuthorship W1631475273A5083361673 @default.
• W1631475273 hasBestOaLocation W16314752731 @default.
• W1631475273 hasConcept C104317684 @default.
• W1631475273 hasConcept C11960822 @default.
• W1631475273 hasConcept C120504264 @default.
• W1631475273 hasConcept C184235292 @default.
• W1631475273 hasConcept C185592680 @default.
• W1631475273 hasConcept C29537977 @default.
• W1631475273 hasConcept C30683889 @default.
• W1631475273 hasConcept C552990157 @default.
• W1631475273 hasConcept C55493867 @default.
• W1631475273 hasConcept C83640560 @default.
• W1631475273 hasConcept C86803240 @default.
• W1631475273 hasConcept C87026931 @default.
• W1631475273 hasConcept C95444343 @default.

• next