Matches in SemOpenAlex for { <https://semopenalex.org/work/W1633401484> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W1633401484 endingPage "10849" @default.
- W1633401484 startingPage "10845" @default.
- W1633401484 abstract "Methylenetetrahydrofolate reductase in Clostridium formicoaceticum has been purified to a specific activity of 140 mumol min-1 mg-1 when assayed at 37 degrees C, pH 7.2, in the direction of oxidation of 5-methyltetrahydrofolate with benzyl viologen as electron acceptor. The purified enzyme is judged to be homogeneous by polyacrylamide disc-gel electrophoresis and gel filtration. The enzyme which is an octamer has a molecular weight of about 237,000 and consists of four each of two different subunits having the molecular weights 26,000 and 35,000. The octameric enzyme contains per mol 15.2 +/- 0.3 iron, 2.3 +/- 0.2 zinc, 19.5 +/- 1.3 acid-labile sulfur, and 1.7 FAD. The UV-visible absorbance spectrum has a peak at 385 nm and a shoulder at 430 nm and is that of a flavoprotein containing iron-sulfur centers. The reductase, which is sensitive to oxygen, must be handled anaerobically and is stabilized by 2 mM dithionite. It catalyzes the reduction of methylene blue, menadione, benzyl viologen, rubredoxin, and FAD with 5-methyltetrahydrofolate and the oxidation of reduced ferredoxin and FADH2 with 5,10-methylenetetrahydrofolate. No activity was observed with pyridine nucleotides. It is suggested that the physiologically important reaction catalyzed by the enzyme is the reduced ferredoxin-dependent reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate." @default.
- W1633401484 created "2016-06-24" @default.
- W1633401484 creator A5047675365 @default.
- W1633401484 creator A5048391102 @default.
- W1633401484 date "1984-09-01" @default.
- W1633401484 modified "2023-09-30" @default.
- W1633401484 title "Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum." @default.
- W1633401484 cites W1443021588 @default.
- W1633401484 cites W1493170688 @default.
- W1633401484 cites W1519541902 @default.
- W1633401484 cites W1531416894 @default.
- W1633401484 cites W1534622291 @default.
- W1633401484 cites W1544722768 @default.
- W1633401484 cites W1553061141 @default.
- W1633401484 cites W1556964559 @default.
- W1633401484 cites W1558499662 @default.
- W1633401484 cites W1566514223 @default.
- W1633401484 cites W1573383902 @default.
- W1633401484 cites W1596802196 @default.
- W1633401484 cites W1607046039 @default.
- W1633401484 cites W1640038695 @default.
- W1633401484 cites W1766118319 @default.
- W1633401484 cites W1769953098 @default.
- W1633401484 cites W2005926878 @default.
- W1633401484 cites W2038345597 @default.
- W1633401484 cites W2044024099 @default.
- W1633401484 cites W2050148786 @default.
- W1633401484 cites W2070307502 @default.
- W1633401484 cites W2079448948 @default.
- W1633401484 cites W2082877976 @default.
- W1633401484 cites W2084870396 @default.
- W1633401484 cites W2103577920 @default.
- W1633401484 cites W2111301002 @default.
- W1633401484 cites W2138604440 @default.
- W1633401484 cites W3119999078 @default.
- W1633401484 doi "https://doi.org/10.1016/s0021-9258(18)90590-9" @default.
- W1633401484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6381490" @default.
- W1633401484 hasPublicationYear "1984" @default.
- W1633401484 type Work @default.
- W1633401484 sameAs 1633401484 @default.
- W1633401484 citedByCount "82" @default.
- W1633401484 countsByYear W16334014842012 @default.
- W1633401484 countsByYear W16334014842013 @default.
- W1633401484 countsByYear W16334014842014 @default.
- W1633401484 countsByYear W16334014842015 @default.
- W1633401484 countsByYear W16334014842016 @default.
- W1633401484 countsByYear W16334014842018 @default.
- W1633401484 countsByYear W16334014842019 @default.
- W1633401484 countsByYear W16334014842020 @default.
- W1633401484 countsByYear W16334014842021 @default.
- W1633401484 countsByYear W16334014842022 @default.
- W1633401484 countsByYear W16334014842023 @default.
- W1633401484 crossrefType "journal-article" @default.
- W1633401484 hasAuthorship W1633401484A5047675365 @default.
- W1633401484 hasAuthorship W1633401484A5048391102 @default.
- W1633401484 hasBestOaLocation W16334014841 @default.
- W1633401484 hasConcept C104317684 @default.
- W1633401484 hasConcept C134651460 @default.
- W1633401484 hasConcept C135763542 @default.
- W1633401484 hasConcept C178790620 @default.
- W1633401484 hasConcept C181199279 @default.
- W1633401484 hasConcept C185592680 @default.
- W1633401484 hasConcept C43563269 @default.
- W1633401484 hasConcept C518881349 @default.
- W1633401484 hasConcept C55493867 @default.
- W1633401484 hasConcept C71995715 @default.
- W1633401484 hasConceptScore W1633401484C104317684 @default.
- W1633401484 hasConceptScore W1633401484C134651460 @default.
- W1633401484 hasConceptScore W1633401484C135763542 @default.
- W1633401484 hasConceptScore W1633401484C178790620 @default.
- W1633401484 hasConceptScore W1633401484C181199279 @default.
- W1633401484 hasConceptScore W1633401484C185592680 @default.
- W1633401484 hasConceptScore W1633401484C43563269 @default.
- W1633401484 hasConceptScore W1633401484C518881349 @default.
- W1633401484 hasConceptScore W1633401484C55493867 @default.
- W1633401484 hasConceptScore W1633401484C71995715 @default.
- W1633401484 hasIssue "17" @default.
- W1633401484 hasLocation W16334014841 @default.
- W1633401484 hasOpenAccess W1633401484 @default.
- W1633401484 hasPrimaryLocation W16334014841 @default.
- W1633401484 hasRelatedWork W1539501970 @default.
- W1633401484 hasRelatedWork W1660328121 @default.
- W1633401484 hasRelatedWork W1963499417 @default.
- W1633401484 hasRelatedWork W2017037513 @default.
- W1633401484 hasRelatedWork W2053393915 @default.
- W1633401484 hasRelatedWork W2082207917 @default.
- W1633401484 hasRelatedWork W2109838107 @default.
- W1633401484 hasRelatedWork W2138227889 @default.
- W1633401484 hasRelatedWork W3125997658 @default.
- W1633401484 hasRelatedWork W3176317270 @default.
- W1633401484 hasVolume "259" @default.
- W1633401484 isParatext "false" @default.
- W1633401484 isRetracted "false" @default.
- W1633401484 magId "1633401484" @default.
- W1633401484 workType "article" @default.