FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1633991623> ?p ?o ?g. }

• W1633991623 endingPage "912" @default.
• W1633991623 startingPage "904" @default.
• W1633991623 abstract "The expression of a retroviral vector with the Moloney murine leukemia virus (Mo-MuLV) long terminal repeat (LTR) promoter after integration into the genome of murine fibroblast cell lines was monitored with the Escherichia coli-derived beta-galactosidase (beta-gal) gene as the reporter. Monoclonal cell lines derived after retroviral infection exhibited a marked heterogeneity in their expression of the reporter gene. We studied two monoclonal cell lines with a single unrearranged copy of the vector provirus integrated into their genome. The first, BB10, expressed the marker enzyme in only 8% of its cell population, whereas in the second, BB16, beta-gal expression could be detected in over 98% of the cells. Treatment of BB10 with the DNA-demethylating agent 5-azacytidine raised the number of beta-gal-positive cells to over 60%. Transfection experiments showed that the Mo-MuLV LTR promoter-enhancer is potentially fully functional in both the BB10 and BB16 cell lines. The inactivated provirus from BB10 cells was cloned and subsequently used to generate retrovirus stocks. The promoter-enhancer activity of its LTR after infection with these BB10-derived viruses showed a variation similar to that of the original virus stocks. Our data showed that (1) inactivation of the Mo-MuLV LTR is a frequent event in murine fibroblast cell lines, (2) inactivation is associated with de novo methylation of cytidine residues, (3) the frequency of inactivation of the provirus must be determined by its chromosomal position, (4) the process of methylation of sequences within the LTR is not necessarily the same as the transcription-repression mechanism that is operating in undifferentiated embryonal carcinoma cells." @default.
• W1633991623 created "2016-06-24" @default.
• W1633991623 creator A5005383720 @default.
• W1633991623 creator A5011699454 @default.
• W1633991623 creator A5044237329 @default.
• W1633991623 creator A5044505683 @default.
• W1633991623 creator A5065286998 @default.
• W1633991623 date "1991-02-01" @default.
• W1633991623 modified "2023-09-26" @default.
• W1633991623 title "Inactivation of the Moloney murine leukemia virus long terminal repeat in murine fibroblast cell lines is associated with methylation and dependent on its chromosomal position" @default.
• W1633991623 cites W119651583 @default.
• W1633991623 cites W1484151854 @default.
• W1633991623 cites W1540415460 @default.
• W1633991623 cites W1548710060 @default.
• W1633991623 cites W1564397880 @default.
• W1633991623 cites W1568345766 @default.
• W1633991623 cites W1600900798 @default.
• W1633991623 cites W1749054014 @default.
• W1633991623 cites W1759752688 @default.
• W1633991623 cites W1801420105 @default.
• W1633991623 cites W1818877461 @default.
• W1633991623 cites W1868517480 @default.
• W1633991623 cites W1942399286 @default.
• W1633991623 cites W1942940126 @default.
• W1633991623 cites W1947669382 @default.
• W1633991623 cites W1965372133 @default.
• W1633991623 cites W1971502404 @default.
• W1633991623 cites W1974950688 @default.
• W1633991623 cites W1987547326 @default.
• W1633991623 cites W1997110527 @default.
• W1633991623 cites W2002639116 @default.
• W1633991623 cites W2008666823 @default.
• W1633991623 cites W2009701813 @default.
• W1633991623 cites W2014669590 @default.
• W1633991623 cites W2018117114 @default.
• W1633991623 cites W2021783595 @default.
• W1633991623 cites W2022205235 @default.
• W1633991623 cites W2024577717 @default.
• W1633991623 cites W2027167740 @default.
• W1633991623 cites W2041286134 @default.
• W1633991623 cites W2042781120 @default.
• W1633991623 cites W2045679225 @default.
• W1633991623 cites W2051806824 @default.
• W1633991623 cites W2054217735 @default.
• W1633991623 cites W2062901218 @default.
• W1633991623 cites W2066896666 @default.
• W1633991623 cites W2069925586 @default.
• W1633991623 cites W2072606138 @default.
• W1633991623 cites W2076355219 @default.
• W1633991623 cites W2088171363 @default.
• W1633991623 cites W2088223280 @default.
• W1633991623 cites W2088420183 @default.
• W1633991623 cites W2118299921 @default.
• W1633991623 cites W2137192579 @default.
• W1633991623 cites W2141842402 @default.
• W1633991623 cites W2143319558 @default.
• W1633991623 cites W2145616979 @default.
• W1633991623 cites W2146033027 @default.
• W1633991623 cites W2153952639 @default.
• W1633991623 cites W2270657113 @default.
• W1633991623 cites W2317047222 @default.
• W1633991623 cites W2461659905 @default.
• W1633991623 doi "https://doi.org/10.1128/jvi.65.2.904-912.1991" @default.
• W1633991623 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/239831" @default.
• W1633991623 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1702844" @default.
• W1633991623 hasPublicationYear "1991" @default.
• W1633991623 type Work @default.
• W1633991623 sameAs 1633991623 @default.
• W1633991623 citedByCount "157" @default.
• W1633991623 countsByYear W16339916232012 @default.
• W1633991623 countsByYear W16339916232013 @default.
• W1633991623 countsByYear W16339916232014 @default.
• W1633991623 countsByYear W16339916232018 @default.
• W1633991623 crossrefType "journal-article" @default.
• W1633991623 hasAuthorship W1633991623A5005383720 @default.
• W1633991623 hasAuthorship W1633991623A5011699454 @default.
• W1633991623 hasAuthorship W1633991623A5044237329 @default.
• W1633991623 hasAuthorship W1633991623A5044505683 @default.
• W1633991623 hasAuthorship W1633991623A5065286998 @default.
• W1633991623 hasBestOaLocation W16339916231 @default.
• W1633991623 hasConcept C102906071 @default.
• W1633991623 hasConcept C104317684 @default.
• W1633991623 hasConcept C111936080 @default.
• W1633991623 hasConcept C141231307 @default.
• W1633991623 hasConcept C150194340 @default.
• W1633991623 hasConcept C153911025 @default.
• W1633991623 hasConcept C159047783 @default.
• W1633991623 hasConcept C199491388 @default.
• W1633991623 hasConcept C2522874641 @default.
• W1633991623 hasConcept C2778431562 @default.
• W1633991623 hasConcept C2779562191 @default.
• W1633991623 hasConcept C54009773 @default.
• W1633991623 hasConcept C54355233 @default.
• W1633991623 hasConcept C81885089 @default.
• W1633991623 hasConcept C86803240 @default.
• W1633991623 hasConceptScore W1633991623C102906071 @default.
• W1633991623 hasConceptScore W1633991623C104317684 @default.
• W1633991623 hasConceptScore W1633991623C111936080 @default.

• next