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• W1634213771 abstract "Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced by Bacillus subtilis 168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture of B. subtilis 168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved." @default.
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• W1634213771 date "2015-01-01" @default.
• W1634213771 modified "2023-09-24" @default.
• W1634213771 title "Improved Production of Sublancin 168 Biosynthesized by<i>Bacillus subtilis</i>168 Using Chemometric Methodology and Statistical Experimental Designs" @default.
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• W1634213771 doi "https://doi.org/10.1155/2015/687915" @default.
• W1634213771 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4538419" @default.
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