Matches in SemOpenAlex for { <https://semopenalex.org/work/W1635567380> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1635567380 endingPage "902" @default.
- W1635567380 startingPage "5893" @default.
- W1635567380 abstract "A human gliosarcoma culture was characterized from the time of inception to the time of establishment of the cell line (SF-539 BT). Immunohistochemical analysis of the original tumor showed 2 distinct regions of cells. The gliomatous regions were identified by immunostains for glial fibrillary acidic protein and the sarcomatous regions by immunostains for laminin, collagen type IV, procollagen type III, and fibronectin. In early-passage culture, both types of cells maintained their characteristic immunohistochemical profiles; however, after the fourth subcultivation in monolayer culture, no cells expressing glial fibrillary acidic protein could be identified. All cells had become morphologically uniform and expressed laminin, collagen type IV, procollagen type III, and fibronectin only. The immunostaining profile of clones grown in soft agar was similar to that of cells in monolayer culture. At establishment, SF-539 BT has a saturation density of 1.3 X 10(6) cells/25 sq cm, a doubling time of 32 h, and a plating efficiency of 22% in monolayer culture. The tumor cell line is resistant to 1,3-bis(2-chloroethyl)-1-nitrosourea, has an abnormal karyotype, grows anchorage independently, and forms a tumor that most closely resembles a spindle cell sarcoma in athymic mice. Its ultrastructure in monolayer culture consists of large cells with an expanded rough endoplasmic reticulum and abundant multivesicular bodies; in athymic mice, extracellular collagen fiber formation is prominent. DEAE-cellulose chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cultures labeled with [3H] proline demonstrated interstitial collagen formation. We conclude that the cell line at establishment is a collagen-producing spindle cell sarcoma that resembles the sarcomatous regions of the original mixed tumor. Further cell separation and characterization studies are needed to determine the pathogenesis of mixed tumors such as gliosarcoma." @default.
- W1635567380 created "2016-06-24" @default.
- W1635567380 creator A5001979226 @default.
- W1635567380 creator A5015818175 @default.
- W1635567380 creator A5025236416 @default.
- W1635567380 creator A5054639277 @default.
- W1635567380 creator A5058287297 @default.
- W1635567380 creator A5059698282 @default.
- W1635567380 creator A5065718203 @default.
- W1635567380 creator A5067215084 @default.
- W1635567380 creator A5088884326 @default.
- W1635567380 date "1986-11-01" @default.
- W1635567380 modified "2023-10-13" @default.
- W1635567380 title "Establishment and characterization of a cell line from a human gliosarcoma." @default.
- W1635567380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3019542" @default.
- W1635567380 hasPublicationYear "1986" @default.
- W1635567380 type Work @default.
- W1635567380 sameAs 1635567380 @default.
- W1635567380 citedByCount "20" @default.
- W1635567380 countsByYear W16355673802012 @default.
- W1635567380 countsByYear W16355673802014 @default.
- W1635567380 countsByYear W16355673802015 @default.
- W1635567380 crossrefType "journal-article" @default.
- W1635567380 hasAuthorship W1635567380A5001979226 @default.
- W1635567380 hasAuthorship W1635567380A5015818175 @default.
- W1635567380 hasAuthorship W1635567380A5025236416 @default.
- W1635567380 hasAuthorship W1635567380A5054639277 @default.
- W1635567380 hasAuthorship W1635567380A5058287297 @default.
- W1635567380 hasAuthorship W1635567380A5059698282 @default.
- W1635567380 hasAuthorship W1635567380A5065718203 @default.
- W1635567380 hasAuthorship W1635567380A5067215084 @default.
- W1635567380 hasAuthorship W1635567380A5088884326 @default.
- W1635567380 hasConcept C142724271 @default.
- W1635567380 hasConcept C1491633281 @default.
- W1635567380 hasConcept C153911025 @default.
- W1635567380 hasConcept C203014093 @default.
- W1635567380 hasConcept C204232928 @default.
- W1635567380 hasConcept C2779814568 @default.
- W1635567380 hasConcept C2780495277 @default.
- W1635567380 hasConcept C4224716 @default.
- W1635567380 hasConcept C54355233 @default.
- W1635567380 hasConcept C55493867 @default.
- W1635567380 hasConcept C71924100 @default.
- W1635567380 hasConcept C81885089 @default.
- W1635567380 hasConcept C86492073 @default.
- W1635567380 hasConcept C86803240 @default.
- W1635567380 hasConceptScore W1635567380C142724271 @default.
- W1635567380 hasConceptScore W1635567380C1491633281 @default.
- W1635567380 hasConceptScore W1635567380C153911025 @default.
- W1635567380 hasConceptScore W1635567380C203014093 @default.
- W1635567380 hasConceptScore W1635567380C204232928 @default.
- W1635567380 hasConceptScore W1635567380C2779814568 @default.
- W1635567380 hasConceptScore W1635567380C2780495277 @default.
- W1635567380 hasConceptScore W1635567380C4224716 @default.
- W1635567380 hasConceptScore W1635567380C54355233 @default.
- W1635567380 hasConceptScore W1635567380C55493867 @default.
- W1635567380 hasConceptScore W1635567380C71924100 @default.
- W1635567380 hasConceptScore W1635567380C81885089 @default.
- W1635567380 hasConceptScore W1635567380C86492073 @default.
- W1635567380 hasConceptScore W1635567380C86803240 @default.
- W1635567380 hasIssue "11" @default.
- W1635567380 hasLocation W16355673801 @default.
- W1635567380 hasOpenAccess W1635567380 @default.
- W1635567380 hasPrimaryLocation W16355673801 @default.
- W1635567380 hasRelatedWork W1524143295 @default.
- W1635567380 hasRelatedWork W1584625755 @default.
- W1635567380 hasRelatedWork W1635567380 @default.
- W1635567380 hasRelatedWork W1982174029 @default.
- W1635567380 hasRelatedWork W1987669338 @default.
- W1635567380 hasRelatedWork W2027871560 @default.
- W1635567380 hasRelatedWork W2118468425 @default.
- W1635567380 hasRelatedWork W2439764154 @default.
- W1635567380 hasRelatedWork W2473189367 @default.
- W1635567380 hasRelatedWork W2182517647 @default.
- W1635567380 hasVolume "46" @default.
- W1635567380 isParatext "false" @default.
- W1635567380 isRetracted "false" @default.
- W1635567380 magId "1635567380" @default.
- W1635567380 workType "article" @default.