Matches in SemOpenAlex for { <https://semopenalex.org/work/W1636691082> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1636691082 endingPage "85" @default.
- W1636691082 startingPage "77" @default.
- W1636691082 abstract "Transgenic systems are invaluable in studies of function of retinoic acids (RAs). The spatial-temporal distribution of RAs within the embryo or a given tissue is difficult to assess because it involves laborious and tissue-disrupting analytical methods such as high-performance liquid chromatography (HPLC). As an alternative, relative RA concentrations can be measured in situ using RA-inducible promoter sequences fused to a reporter gene. Transgenic approaches may be also used to identify novel RA-dependent genes. Gene traps are used to identify novel genes that are important for development. In this strategy, pluripotent mouse embryonic stem (ES) cells are electroporated with a gene trap vector. The vector consists of a promoterless reporter gene (typically β-galactosidase), preceded by a splice-acceptor site. A selection marker provides means to select clones derived from ES cells that have integrated the construct into their genomes. Alternatively, the β-galactosidase and neomycin phosphotransferase activities can be contained in a single fusion protein. The activity of the reporter gene can be visualized by histological staining. Stained clones contain β-galactosidase-tagged genes, which are expressed in the ES cells. A modification of this technique allows the investigator to identify a group of genes that is inducible by a particular soluble factor." @default.
- W1636691082 created "2016-06-24" @default.
- W1636691082 creator A5002047730 @default.
- W1636691082 creator A5017846615 @default.
- W1636691082 date "1997-01-01" @default.
- W1636691082 modified "2023-10-17" @default.
- W1636691082 title "[7] Use of transgenic mice to study activation of retinoic acid-responsive promoters" @default.
- W1636691082 cites W1976271397 @default.
- W1636691082 cites W1979423222 @default.
- W1636691082 cites W1981412858 @default.
- W1636691082 cites W2005883936 @default.
- W1636691082 cites W2013126973 @default.
- W1636691082 cites W2014721239 @default.
- W1636691082 cites W2025631499 @default.
- W1636691082 cites W2033722975 @default.
- W1636691082 cites W2051663421 @default.
- W1636691082 cites W2053520712 @default.
- W1636691082 cites W206917024 @default.
- W1636691082 cites W2082103215 @default.
- W1636691082 cites W2091181040 @default.
- W1636691082 cites W2091929104 @default.
- W1636691082 cites W2189084452 @default.
- W1636691082 cites W2198062791 @default.
- W1636691082 cites W2207516260 @default.
- W1636691082 doi "https://doi.org/10.1016/s0076-6879(97)82097-6" @default.
- W1636691082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9330278" @default.
- W1636691082 hasPublicationYear "1997" @default.
- W1636691082 type Work @default.
- W1636691082 sameAs 1636691082 @default.
- W1636691082 citedByCount "0" @default.
- W1636691082 crossrefType "book-chapter" @default.
- W1636691082 hasAuthorship W1636691082A5002047730 @default.
- W1636691082 hasAuthorship W1636691082A5017846615 @default.
- W1636691082 hasConcept C101762097 @default.
- W1636691082 hasConcept C102230213 @default.
- W1636691082 hasConcept C104317684 @default.
- W1636691082 hasConcept C111829193 @default.
- W1636691082 hasConcept C141231307 @default.
- W1636691082 hasConcept C150194340 @default.
- W1636691082 hasConcept C153911025 @default.
- W1636691082 hasConcept C161733203 @default.
- W1636691082 hasConcept C173764188 @default.
- W1636691082 hasConcept C182901222 @default.
- W1636691082 hasConcept C2781121885 @default.
- W1636691082 hasConcept C54355233 @default.
- W1636691082 hasConcept C86803240 @default.
- W1636691082 hasConcept C95444343 @default.
- W1636691082 hasConceptScore W1636691082C101762097 @default.
- W1636691082 hasConceptScore W1636691082C102230213 @default.
- W1636691082 hasConceptScore W1636691082C104317684 @default.
- W1636691082 hasConceptScore W1636691082C111829193 @default.
- W1636691082 hasConceptScore W1636691082C141231307 @default.
- W1636691082 hasConceptScore W1636691082C150194340 @default.
- W1636691082 hasConceptScore W1636691082C153911025 @default.
- W1636691082 hasConceptScore W1636691082C161733203 @default.
- W1636691082 hasConceptScore W1636691082C173764188 @default.
- W1636691082 hasConceptScore W1636691082C182901222 @default.
- W1636691082 hasConceptScore W1636691082C2781121885 @default.
- W1636691082 hasConceptScore W1636691082C54355233 @default.
- W1636691082 hasConceptScore W1636691082C86803240 @default.
- W1636691082 hasConceptScore W1636691082C95444343 @default.
- W1636691082 hasLocation W16366910821 @default.
- W1636691082 hasLocation W16366910822 @default.
- W1636691082 hasOpenAccess W1636691082 @default.
- W1636691082 hasPrimaryLocation W16366910821 @default.
- W1636691082 hasRelatedWork W151795848 @default.
- W1636691082 hasRelatedWork W1636691082 @default.
- W1636691082 hasRelatedWork W2041247988 @default.
- W1636691082 hasRelatedWork W2120666391 @default.
- W1636691082 hasRelatedWork W2143108234 @default.
- W1636691082 hasRelatedWork W2153071879 @default.
- W1636691082 hasRelatedWork W2209181031 @default.
- W1636691082 hasRelatedWork W2376650959 @default.
- W1636691082 hasRelatedWork W2378271209 @default.
- W1636691082 hasRelatedWork W2953475452 @default.
- W1636691082 isParatext "false" @default.
- W1636691082 isRetracted "false" @default.
- W1636691082 magId "1636691082" @default.
- W1636691082 workType "book-chapter" @default.