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- W1637776987 abstract "The fungus Neurospora crassa represents a eukaryotic cell with high biosynthetic activities. Cell mass doubles in 2–4 hours during exponential growth even in simple salt media with sucrose as the sole carbon source. The microorganism forms a mycelium of long hyphae during vegetative growth. The mitochondria can be isolated under relatively gentle conditions since a few breaks in the threadlike hyphae are sufficient to cause the outflow of the organelles. The chapter describes two methods for the physical disruption of the hyphae: (1) the cells are opened in a grind mill between two rotating corundum disks. This is a continuous and fast procedure and allows large- and small-scale preparations of mitochondria. (2) Hyphae are ground with sand in a mortar and pestle. This procedure can be applied to microscale preparations of mitochondria starting with minute amounts of cells. It also provides an overview on other procedures for the isolation of Neurospora mitochondria after the physical disruption or the enzymatic degradation of the cell wall." @default.
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- W1637776987 date "1979-01-01" @default.
- W1637776987 modified "2023-10-14" @default.
- W1637776987 title "[18] Preparation of Neurospora crassa mitochondria" @default.
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- W1637776987 doi "https://doi.org/10.1016/0076-6879(79)55020-4" @default.
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