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• W1639307940 abstract "Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in the production of haemocin were detected. This strategy provides an efficient mechanism for the insertional mutagenesis of H. influenzae." @default.
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• W1639307940 date "1991-02-01" @default.
• W1639307940 modified "2023-09-27" @default.
• W1639307940 title "A novel approach to insertional mutagenesis of Haemophilus influenzae" @default.
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• W1639307940 doi "https://doi.org/10.1128/jb.173.4.1561-1564.1991" @default.
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