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- W1640511970 abstract "Publisher Summary This chapter provides an overview of comprehensive transcriptional mapping, as it would be applied to the genomes of mammals or other higher vertebrates. In this context, the chapter discusses four techniques for gene identification that have demonstrated their utility for reliable analysis of large genomic regions: dbEST (database of expressed sequence tags) analysis, cDNA selection, exon trapping, and genomic sequence analysis. Generally developed for analysis of mammalian genomes, these techniques are applicable to any genome where the presence of introns, large intergenic distances, and/or repetitive DNA significantly reduces the proportion of coding to noncoding sequence. The chapter describes the general advantages and disadvantages of each technique. The chapter discusses the preliminary considerations for defining the magnitude and scope of the gene search and the necessary resources for beginning the gene search." @default.
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- W1640511970 date "1999-01-01" @default.
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- W1640511970 title "[10] Saturation identification of coding sequences in genomic DNA" @default.
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- W1640511970 doi "https://doi.org/10.1016/s0076-6879(99)03012-8" @default.
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