FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1640761323> ?p ?o ?g. }

• W1640761323 endingPage "3627" @default.
• W1640761323 startingPage "3618" @default.
• W1640761323 abstract "We have investigated the temporal and spatial regulation of spoIIM, a gene of Bacillus subtilis whose product is required for complete septum migration and engulfment of the forespore compartment during sporulation. The spoIIM promoter was found to become active about 2 h after the initiation of sporulation. The effects of mutations on the expression of a spoIIM-lacZ fusion were most consistent with its utilization by sigma-E-associated RNA polymerase (E sigma E). A unique 5' end of the in vivo spoIIM transcript was detected by primer extension analysis and was determined to initiate at the appropriate distance from a sequence conforming very closely to the consensus for genes transcribed by E sigma E. A partially purified preparation of E sigma E produced a transcript in vitro that initiated at the same nucleotide as the primer extension product generated from in vivo RNA. Ectopic induction of sigma E synthesis during growth resulted in the immediate and strong expression of a spoIIM-lacZ fusion, but an identical fusion was completely unresponsive to induced synthesis of either sigma F or sigma G under similar conditions. The results of plasmid integration-excision experiments in which the spoIIM gene was reversibly disrupted by a temperature-sensitive integrational vector suggested that spoIIM expression is required in the forespore compartment, but direct examination of subcellular fractions enriched for mother cell or forespore material indicated that spoIIM expression cannot be confined to the forespore. We conclude that spoIIM is a member of the sigma E regulon and that it may be transcribed exclusively by E sigma E. We discuss the implications of this conclusion for models in which activation of sigma E in the mother cell is proposed to be a part of the mechanism responsible for initiating separate programs of gene activity in the two sporangium compartments." @default.
• W1640761323 created "2016-06-24" @default.
• W1640761323 creator A5027086241 @default.
• W1640761323 creator A5047496374 @default.
• W1640761323 date "1993-06-01" @default.
• W1640761323 modified "2023-09-23" @default.
• W1640761323 title "Evidence that the spoIIM gene of Bacillus subtilis is transcribed by RNA polymerase associated with sigma E" @default.
• W1640761323 cites W1480163145 @default.
• W1640761323 cites W1497811962 @default.
• W1640761323 cites W1499595340 @default.
• W1640761323 cites W1503373278 @default.
• W1640761323 cites W1508053117 @default.
• W1640761323 cites W1522024616 @default.
• W1640761323 cites W1523879423 @default.
• W1640761323 cites W1534138868 @default.
• W1640761323 cites W1539601278 @default.
• W1640761323 cites W1541235276 @default.
• W1640761323 cites W1552212939 @default.
• W1640761323 cites W1567173897 @default.
• W1640761323 cites W1574598042 @default.
• W1640761323 cites W1582834972 @default.
• W1640761323 cites W1601687245 @default.
• W1640761323 cites W1605139332 @default.
• W1640761323 cites W1645310954 @default.
• W1640761323 cites W1658229959 @default.
• W1640761323 cites W1678194650 @default.
• W1640761323 cites W1712125974 @default.
• W1640761323 cites W1720341817 @default.
• W1640761323 cites W1737952852 @default.
• W1640761323 cites W1762767673 @default.
• W1640761323 cites W1797965567 @default.
• W1640761323 cites W1831228939 @default.
• W1640761323 cites W1897953158 @default.
• W1640761323 cites W1927731921 @default.
• W1640761323 cites W1931964389 @default.
• W1640761323 cites W1945765509 @default.
• W1640761323 cites W1952635464 @default.
• W1640761323 cites W1958186528 @default.
• W1640761323 cites W1964462346 @default.
• W1640761323 cites W1968477104 @default.
• W1640761323 cites W1972439457 @default.
• W1640761323 cites W1977588865 @default.
• W1640761323 cites W1983845534 @default.
• W1640761323 cites W1984497246 @default.
• W1640761323 cites W1986725509 @default.
• W1640761323 cites W1997012629 @default.
• W1640761323 cites W1997971408 @default.
• W1640761323 cites W1998138820 @default.
• W1640761323 cites W2000967117 @default.
• W1640761323 cites W2019410656 @default.
• W1640761323 cites W2022474924 @default.
• W1640761323 cites W2028622989 @default.
• W1640761323 cites W2030535034 @default.
• W1640761323 cites W2030911977 @default.
• W1640761323 cites W2034159958 @default.
• W1640761323 cites W2037467204 @default.
• W1640761323 cites W2042248604 @default.
• W1640761323 cites W2045050792 @default.
• W1640761323 cites W2052622095 @default.
• W1640761323 cites W2053149333 @default.
• W1640761323 cites W2054096764 @default.
• W1640761323 cites W2075244297 @default.
• W1640761323 cites W2076342303 @default.
• W1640761323 cites W2085686817 @default.
• W1640761323 cites W2089433978 @default.
• W1640761323 cites W2089526387 @default.
• W1640761323 cites W2092817937 @default.
• W1640761323 cites W2100403047 @default.
• W1640761323 cites W2103677208 @default.
• W1640761323 cites W2103759161 @default.
• W1640761323 cites W2107420011 @default.
• W1640761323 cites W2107647518 @default.
• W1640761323 cites W2111890710 @default.
• W1640761323 cites W2121825059 @default.
• W1640761323 cites W2123812634 @default.
• W1640761323 cites W2127946433 @default.
• W1640761323 cites W2132367233 @default.
• W1640761323 cites W2133133228 @default.
• W1640761323 cites W2139124359 @default.
• W1640761323 cites W2140324276 @default.
• W1640761323 cites W2142069650 @default.
• W1640761323 cites W2145489514 @default.
• W1640761323 cites W2146953954 @default.
• W1640761323 cites W2149516046 @default.
• W1640761323 cites W2156451580 @default.
• W1640761323 cites W2160548348 @default.
• W1640761323 cites W2162113993 @default.
• W1640761323 cites W2171220037 @default.
• W1640761323 cites W2490791623 @default.
• W1640761323 cites W992134897 @default.
• W1640761323 doi "https://doi.org/10.1128/jb.175.11.3618-3627.1993" @default.
• W1640761323 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/204763" @default.
• W1640761323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8501065" @default.
• W1640761323 hasPublicationYear "1993" @default.
• W1640761323 type Work @default.
• W1640761323 sameAs 1640761323 @default.
• W1640761323 citedByCount "30" @default.
• W1640761323 countsByYear W16407613232012 @default.

• next