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- W1640985803 abstract "Chromatin can be assembled on circular DNA molecules in vitro using a high-speed supernatant of Xenopus oocytes (oocyte S-1501). The oocyte S-150 provides both the histone proteins and the enzymes used in this complex process, which closely mimics the one observed in vivo when circular DNA molecules are injected into the germinal vesicle. When the procedures described below are followed, the circular DNA is quantitatively assembled into a minichromosome displaying a full complement of regularly spaced nucleosomes. The reaction is very sensitive to dilution of the S-150 and to ionic strength. The procedures detailed below must be followed if native chromatin is to be produced; any changes introduced in this experimental protocol should be carefully evaluated, and their effect should be ascertained by appropriate MNase digestions of the product. Limited amounts of DNA must be used, so as not to exceed the assembly capacity of the oocyte S- 150. To facilitate analysis of small amounts of chromatin, DNA circles labeled with high specific activity 32p may be used." @default.
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- W1640985803 date "1989-01-01" @default.
- W1640985803 modified "2023-10-18" @default.
- W1640985803 title "[29] Assembly of chromatin with oocyte extracts" @default.
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- W1640985803 doi "https://doi.org/10.1016/0076-6879(89)70067-7" @default.
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