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- W1642603977 abstract "Two-dimensional electrophoresis has been used to document changes in nuclear proteins following viral transformation of an epithelial cell line exhibiting differentiation markers. After transformation, these markers are lost, and the cells become tumorigenic and capable of growth in soft agar. A sharp rise in the phosphorylation of histones H1, H2A, and ubiquitinated H2A is seen on transformation, together with the appearance of three phosphorylated proteins that are extractable by perchloric acid and appear related to high mobility group Protein 14, a constituent of active chromatin. Since comparison is made between normal and transformed cells that are each grown to confluence and since there is little difference between their observed growth rates, the changes seen represent intrinsic differences between the cell lines and are thus a direct reflection of the process of transformation." @default.
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- W1642603977 date "1985-12-01" @default.
- W1642603977 modified "2023-09-23" @default.
- W1642603977 title "Changes in nuclear proteins on transformation of rat epithelial thyroid cells by a murine sarcoma retrovirus." @default.
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