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- W164343054 abstract "Oxygen uptake was determined on whole cells for resistant and sensitive haploid and diploid strains in the presence and absence of 10/sup -3/ M CuCl/sub 2/. The resistant haploid and diploid showed no response to the copper while the sensitive haploid and diploid were inhibited in their uptake capacity in the presence of copper. Oxygen uptake studies on isolated mitochondria from each of the four strains showed that oxygen consumption was drastically reduced in each instance. Transmission electron microscopy revealed that cells from each of the four strains grown in media lacking copper were similar in ultrastructure, having well defined nuclei and mitochondria. Cells grown in media containing copper exhibited differences. The sensitive haploid and diploid had no distinct mitochondria nor nuclei. The resistant haploid and diploid displayed well defined nuclei and mitochondria. In addition, the resistant cells showed a darkening of the cell wall. Flame photometer analyses of the cell wall and cell sap for each strain grown in each type of media indicated that the sensitive strains had copper in the cell sap rather than in the cell wall, whereas in the resistant strains, the concentration of copper in the cell wall was slightly less than that inmore » the growth media, with a trace amount present in the cell sap.« less" @default.
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- W164343054 date "1975-01-01" @default.
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- W164343054 title "Copper sensitivity and resistance in Saccharomyces" @default.
- W164343054 hasPublicationYear "1975" @default.
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