Matches in SemOpenAlex for { <https://semopenalex.org/work/W1643749360> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W1643749360 endingPage "2737" @default.
- W1643749360 startingPage "2725" @default.
- W1643749360 abstract "The biosynthesis of two tetrahydropterin intermediates (H4pterin-1 and H4pterin-2), their conversion to tetrahydrobiopterin, and their overall chemical structures are described. A new high performance liquid chromatographic separation of these and other tetrahydropterins is also described. The biosynthesis of tetrahydrobiopterin from dihydroneopterin triphosphate proceeds in the presence of the bovine adrenal medullary biosynthetic enzymes, Mg2+, NADPH. The biosynthesis of H4pterin-2 occurs under identical conditions, and the compound accumulates in the presence of 1 to 10 microM of N-acetylserotonin, an inhibitor of sepiapterin reductase. At higher concentrations of the inhibitor, the synthesis of H4pterin-2 is also inhibited, and H4pterin-1 accumulates. H4pterin-1 also accumulates in the absence of NADPH. In the presence of NADPH the biosynthetic enzymes convert both intermediates to tetrahydrobiopterin at rates which are greater than the rate of conversion of dihydroneopterin triphosphate to tetrahydrobiopterin. Electrochemical, UV/VIS, oxidation, and ionization properties identify the compounds as tetrahydropterins. The side chain structures of the compounds were determined by a combination of chemical means. The structures of the compounds are 6R-(1',2'-dioxopropyl)-tetrahydropterin (H4pterin-1) and 6R-(L-1'-hydroxy-2'-oxopropyl)-tetrahydropterin (H4pterin-2). The data indicate that the biosynthesis of tetrahydrobiopterin from dihydroneopterin triphosphate proceeds in three steps: 1) formation of H4pterin-1 in the presence of Mg2+, 2) NADPH-dependent conversion of H4pterin-1 to H4pterin-2, and 3) NADPH-dependent conversion of H4pterin-2 to tetrahydrobiopterin." @default.
- W1643749360 created "2016-06-24" @default.
- W1643749360 creator A5004918159 @default.
- W1643749360 creator A5052519692 @default.
- W1643749360 date "1986-02-01" @default.
- W1643749360 modified "2023-09-27" @default.
- W1643749360 title "Synthesis, utilization, and structure of the tetrahydropterin intermediates in the bovine adrenal medullary de novo biosynthesis of tetrahydrobiopterin." @default.
- W1643749360 cites W118921897 @default.
- W1643749360 cites W121486199 @default.
- W1643749360 cites W1494854751 @default.
- W1643749360 cites W1510182625 @default.
- W1643749360 cites W1597200622 @default.
- W1643749360 cites W1602902311 @default.
- W1643749360 cites W1669877415 @default.
- W1643749360 cites W1966490337 @default.
- W1643749360 cites W1971415479 @default.
- W1643749360 cites W1972182855 @default.
- W1643749360 cites W1978429147 @default.
- W1643749360 cites W1982941131 @default.
- W1643749360 cites W1997175049 @default.
- W1643749360 cites W2011302339 @default.
- W1643749360 cites W2015445328 @default.
- W1643749360 cites W2020013084 @default.
- W1643749360 cites W2026213097 @default.
- W1643749360 cites W2031934322 @default.
- W1643749360 cites W2036463932 @default.
- W1643749360 cites W2056866147 @default.
- W1643749360 cites W2057757962 @default.
- W1643749360 cites W2069661716 @default.
- W1643749360 cites W2071604148 @default.
- W1643749360 cites W2081206128 @default.
- W1643749360 cites W2082474253 @default.
- W1643749360 cites W2086955825 @default.
- W1643749360 cites W2094644392 @default.
- W1643749360 cites W2117846724 @default.
- W1643749360 cites W2166737442 @default.
- W1643749360 cites W2176832201 @default.
- W1643749360 cites W970978831 @default.
- W1643749360 doi "https://doi.org/10.1016/s0021-9258(17)35846-5" @default.
- W1643749360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3949745" @default.
- W1643749360 hasPublicationYear "1986" @default.
- W1643749360 type Work @default.
- W1643749360 sameAs 1643749360 @default.
- W1643749360 citedByCount "43" @default.
- W1643749360 crossrefType "journal-article" @default.
- W1643749360 hasAuthorship W1643749360A5004918159 @default.
- W1643749360 hasAuthorship W1643749360A5052519692 @default.
- W1643749360 hasBestOaLocation W16437493601 @default.
- W1643749360 hasConcept C126322002 @default.
- W1643749360 hasConcept C181199279 @default.
- W1643749360 hasConcept C185592680 @default.
- W1643749360 hasConcept C195121873 @default.
- W1643749360 hasConcept C197957613 @default.
- W1643749360 hasConcept C2776145790 @default.
- W1643749360 hasConcept C2777451243 @default.
- W1643749360 hasConcept C553450214 @default.
- W1643749360 hasConcept C55493867 @default.
- W1643749360 hasConcept C71924100 @default.
- W1643749360 hasConcept C88742014 @default.
- W1643749360 hasConceptScore W1643749360C126322002 @default.
- W1643749360 hasConceptScore W1643749360C181199279 @default.
- W1643749360 hasConceptScore W1643749360C185592680 @default.
- W1643749360 hasConceptScore W1643749360C195121873 @default.
- W1643749360 hasConceptScore W1643749360C197957613 @default.
- W1643749360 hasConceptScore W1643749360C2776145790 @default.
- W1643749360 hasConceptScore W1643749360C2777451243 @default.
- W1643749360 hasConceptScore W1643749360C553450214 @default.
- W1643749360 hasConceptScore W1643749360C55493867 @default.
- W1643749360 hasConceptScore W1643749360C71924100 @default.
- W1643749360 hasConceptScore W1643749360C88742014 @default.
- W1643749360 hasIssue "6" @default.
- W1643749360 hasLocation W16437493601 @default.
- W1643749360 hasOpenAccess W1643749360 @default.
- W1643749360 hasPrimaryLocation W16437493601 @default.
- W1643749360 hasRelatedWork W1643749360 @default.
- W1643749360 hasRelatedWork W1970817041 @default.
- W1643749360 hasRelatedWork W2010387767 @default.
- W1643749360 hasRelatedWork W2056866147 @default.
- W1643749360 hasRelatedWork W205703808 @default.
- W1643749360 hasRelatedWork W2079182225 @default.
- W1643749360 hasRelatedWork W2081206128 @default.
- W1643749360 hasRelatedWork W2180844056 @default.
- W1643749360 hasRelatedWork W56940988 @default.
- W1643749360 hasRelatedWork W960495809 @default.
- W1643749360 hasVolume "261" @default.
- W1643749360 isParatext "false" @default.
- W1643749360 isRetracted "false" @default.
- W1643749360 magId "1643749360" @default.
- W1643749360 workType "article" @default.