Matches in SemOpenAlex for { <https://semopenalex.org/work/W1645671018> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1645671018 endingPage "20" @default.
- W1645671018 startingPage "2615" @default.
- W1645671018 abstract "A tumor surface protein (TSP-180) has been identified on murine lung carcinomas using two monoclonal antibodies (MoAbs) (135-13C and 346-11A). Quantitative analysis of TSP-180 on 3LL variants maintained either in vitro or in vivo indicates that TSP-180 is highly expressed in highly malignant metastatic cells. In reducing conditions, sodium dodecyl sulfate-polyacrylamide gel electrophoresis banding patterns of TSP-180 obtained with MoAb 135-13C from cell lysates of 3LL metastatic cells show three proteins migrating to Mr 204,000, 134,000, and 116,000. In the same experimental conditions MoAb 135-13C precipitates from low metastasizing ones only one band, corresponding to the lower molecular weight (Mr 116,000). All bands of TSP-180 observed in 3LL variants are labeled by lactoperoxidase-catalyzed radioiodination of viable cells, incorporate 32PO4, and contain carbohydrates, as judged by binding to wheat germ agglutinin. These results indicate that all proteins have external exposure on the cell surface and that at least some of TSP-180 proteins could be differentially regulated in different tumor cells (highly metastatic versus low metastatic). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis banding patterns and immunoblots obtained from cell lysates of 3LL variants by using a monoclonal antibody to phosphotyrosine (IG-2) indicate that this MoAb recognizes proteins migrating with molecular weights identical to those reported for TSP-180. Moreover, the immunoblots of solubilized immunocomplex, obtained from cell lysates of 3LL variants by using MoAb 135-13C, demonstrate that MoAb IG-2 specifically reacts with TSP-180 proteins. Experiments undertaken in order to assess if some or all of TSP-180 proteins have tyrosine kinase activity demonstrate that MoAb 135-13C binding to the cell surface induces specific phosphorylation of the Mr 204,000 protein of TSP-180. Phosphoaminoacid analysis of the ligand-induced phosphorylated protein (pp204) demonstrates that this protein is phosphorylated at serine and tyrosine. Results reported lead us to hypothesize that TSP-180 is involved in growth-regulation mechanisms and that its high expression on cells with more malignant phenotype could be responsible for a proliferative advantage of such tumor clones." @default.
- W1645671018 created "2016-06-24" @default.
- W1645671018 creator A5004148079 @default.
- W1645671018 creator A5031142659 @default.
- W1645671018 creator A5054461917 @default.
- W1645671018 creator A5057947254 @default.
- W1645671018 creator A5067282968 @default.
- W1645671018 creator A5076653973 @default.
- W1645671018 date "1989-05-15" @default.
- W1645671018 modified "2023-09-27" @default.
- W1645671018 title "Ligand-induced phosphorylation of a murine tumor surface protein (TSP-180) associated with metastatic phenotype." @default.
- W1645671018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2713845" @default.
- W1645671018 hasPublicationYear "1989" @default.
- W1645671018 type Work @default.
- W1645671018 sameAs 1645671018 @default.
- W1645671018 citedByCount "14" @default.
- W1645671018 countsByYear W16456710182016 @default.
- W1645671018 countsByYear W16456710182017 @default.
- W1645671018 countsByYear W16456710182018 @default.
- W1645671018 countsByYear W16456710182019 @default.
- W1645671018 crossrefType "journal-article" @default.
- W1645671018 hasAuthorship W1645671018A5004148079 @default.
- W1645671018 hasAuthorship W1645671018A5031142659 @default.
- W1645671018 hasAuthorship W1645671018A5054461917 @default.
- W1645671018 hasAuthorship W1645671018A5057947254 @default.
- W1645671018 hasAuthorship W1645671018A5067282968 @default.
- W1645671018 hasAuthorship W1645671018A5076653973 @default.
- W1645671018 hasConcept C101660505 @default.
- W1645671018 hasConcept C116084860 @default.
- W1645671018 hasConcept C1491633281 @default.
- W1645671018 hasConcept C153911025 @default.
- W1645671018 hasConcept C159654299 @default.
- W1645671018 hasConcept C181199279 @default.
- W1645671018 hasConcept C185592680 @default.
- W1645671018 hasConcept C203014093 @default.
- W1645671018 hasConcept C2778913249 @default.
- W1645671018 hasConcept C2780175098 @default.
- W1645671018 hasConcept C2780758401 @default.
- W1645671018 hasConcept C542903549 @default.
- W1645671018 hasConcept C55493867 @default.
- W1645671018 hasConcept C86803240 @default.
- W1645671018 hasConceptScore W1645671018C101660505 @default.
- W1645671018 hasConceptScore W1645671018C116084860 @default.
- W1645671018 hasConceptScore W1645671018C1491633281 @default.
- W1645671018 hasConceptScore W1645671018C153911025 @default.
- W1645671018 hasConceptScore W1645671018C159654299 @default.
- W1645671018 hasConceptScore W1645671018C181199279 @default.
- W1645671018 hasConceptScore W1645671018C185592680 @default.
- W1645671018 hasConceptScore W1645671018C203014093 @default.
- W1645671018 hasConceptScore W1645671018C2778913249 @default.
- W1645671018 hasConceptScore W1645671018C2780175098 @default.
- W1645671018 hasConceptScore W1645671018C2780758401 @default.
- W1645671018 hasConceptScore W1645671018C542903549 @default.
- W1645671018 hasConceptScore W1645671018C55493867 @default.
- W1645671018 hasConceptScore W1645671018C86803240 @default.
- W1645671018 hasIssue "10" @default.
- W1645671018 hasLocation W16456710181 @default.
- W1645671018 hasOpenAccess W1645671018 @default.
- W1645671018 hasPrimaryLocation W16456710181 @default.
- W1645671018 hasRelatedWork W1003977362 @default.
- W1645671018 hasRelatedWork W1550885395 @default.
- W1645671018 hasRelatedWork W1563473939 @default.
- W1645671018 hasRelatedWork W1824695825 @default.
- W1645671018 hasRelatedWork W2008566393 @default.
- W1645671018 hasRelatedWork W2032682781 @default.
- W1645671018 hasRelatedWork W2035337224 @default.
- W1645671018 hasRelatedWork W2051286266 @default.
- W1645671018 hasRelatedWork W2061477200 @default.
- W1645671018 hasRelatedWork W2087326842 @default.
- W1645671018 hasVolume "49" @default.
- W1645671018 isParatext "false" @default.
- W1645671018 isRetracted "false" @default.
- W1645671018 magId "1645671018" @default.
- W1645671018 workType "article" @default.