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• W1647597862 abstract "Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins. To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins. A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities. The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution. Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules." @default.
• W1647597862 created "2016-06-24" @default.
• W1647597862 creator A5035618234 @default.
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• W1647597862 date "2000-09-08" @default.
• W1647597862 modified "2023-10-16" @default.
• W1647597862 title "Printing Proteins as Microarrays for High-Throughput Function Determination" @default.
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• W1647597862 doi "https://doi.org/10.1126/science.289.5485.1760" @default.
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