Matches in SemOpenAlex for { <https://semopenalex.org/work/W1649067458> ?p ?o ?g. }
- W1649067458 endingPage "763" @default.
- W1649067458 startingPage "750" @default.
- W1649067458 abstract "Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs were found to be 2.1, 2.6, and 0.7% virus specific, respectively. Size classes within subcellular fractions were determined by sucrose gradient centrifugation in the presence of dimethyl sulfoxide followed by hybridization. The cytoplasmic fraction contained a 28S size class, which corresponds to the size of virion subunit RNA, and 36S, 23S, and 15 to 18S RNA species. The virus-specific 36S, 23S, and 15 to 18S species but not the 28S RNA were present in both the total and polyadenylic acid-containing polyribosomal RNA. Anti-FeLV gamma globulin bound to rapidly sedimenting polyribosomes, with the peak binding at 400S. The specificity of the binding for nascent virus-specific protein was determined in control experiments that involved mixing polyribosomes with soluble virion proteins, absorption of specific gamma globulin with soluble virion proteins, and puromycin-induced nascent protein release. The R-FeLV cDNA probe hybridized to RNA in two polyribosomal regions (approximately 400 to 450S and 250S) within the polyribosomal gradients before but not after EDTA treatment. The 400 to 450S polyribosomes contained three major peaks of virus-specific RNA at 36S, 23S, and 15 to 18S, whereas the 250S polyribosomes contained predominantly 36S and 15 to 18S RNA. Further experiments suggest that an approximately 36S minor subunit is present in virion RNA." @default.
- W1649067458 created "2016-06-24" @default.
- W1649067458 creator A5032754217 @default.
- W1649067458 creator A5078550136 @default.
- W1649067458 date "1978-03-01" @default.
- W1649067458 modified "2023-09-27" @default.
- W1649067458 title "Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells" @default.
- W1649067458 cites W1513007123 @default.
- W1649067458 cites W1515850643 @default.
- W1649067458 cites W1522622091 @default.
- W1649067458 cites W1522857363 @default.
- W1649067458 cites W1541922629 @default.
- W1649067458 cites W1563081363 @default.
- W1649067458 cites W1569445790 @default.
- W1649067458 cites W1575056372 @default.
- W1649067458 cites W1576239999 @default.
- W1649067458 cites W1582605621 @default.
- W1649067458 cites W1631202616 @default.
- W1649067458 cites W1637744532 @default.
- W1649067458 cites W1657463452 @default.
- W1649067458 cites W1667519583 @default.
- W1649067458 cites W1689612166 @default.
- W1649067458 cites W1701072163 @default.
- W1649067458 cites W1745214696 @default.
- W1649067458 cites W1759452665 @default.
- W1649067458 cites W1765986460 @default.
- W1649067458 cites W1775749144 @default.
- W1649067458 cites W1795036143 @default.
- W1649067458 cites W1803575496 @default.
- W1649067458 cites W1864054986 @default.
- W1649067458 cites W1901567320 @default.
- W1649067458 cites W1928512647 @default.
- W1649067458 cites W1968598595 @default.
- W1649067458 cites W1969322968 @default.
- W1649067458 cites W1971495757 @default.
- W1649067458 cites W1978013460 @default.
- W1649067458 cites W1980600234 @default.
- W1649067458 cites W1981957147 @default.
- W1649067458 cites W1989415062 @default.
- W1649067458 cites W1992893612 @default.
- W1649067458 cites W1997779337 @default.
- W1649067458 cites W1997939648 @default.
- W1649067458 cites W1997989544 @default.
- W1649067458 cites W1999347022 @default.
- W1649067458 cites W2002581213 @default.
- W1649067458 cites W2003572968 @default.
- W1649067458 cites W2006716098 @default.
- W1649067458 cites W2014027374 @default.
- W1649067458 cites W2016896206 @default.
- W1649067458 cites W2018284220 @default.
- W1649067458 cites W2027026276 @default.
- W1649067458 cites W2028321385 @default.
- W1649067458 cites W2038123178 @default.
- W1649067458 cites W2043656337 @default.
- W1649067458 cites W2061970525 @default.
- W1649067458 cites W2063581750 @default.
- W1649067458 cites W2063894433 @default.
- W1649067458 cites W2065485884 @default.
- W1649067458 cites W2066127463 @default.
- W1649067458 cites W2067169105 @default.
- W1649067458 cites W2067906745 @default.
- W1649067458 cites W2069105605 @default.
- W1649067458 cites W2074032747 @default.
- W1649067458 cites W2086201022 @default.
- W1649067458 cites W2088128491 @default.
- W1649067458 cites W2091570267 @default.
- W1649067458 cites W2097529388 @default.
- W1649067458 cites W2135113154 @default.
- W1649067458 cites W1987143974 @default.
- W1649067458 doi "https://doi.org/10.1128/jvi.25.3.750-763.1978" @default.
- W1649067458 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/525968" @default.
- W1649067458 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/205670" @default.
- W1649067458 hasPublicationYear "1978" @default.
- W1649067458 type Work @default.
- W1649067458 sameAs 1649067458 @default.
- W1649067458 citedByCount "7" @default.
- W1649067458 crossrefType "journal-article" @default.
- W1649067458 hasAuthorship W1649067458A5032754217 @default.
- W1649067458 hasAuthorship W1649067458A5078550136 @default.
- W1649067458 hasBestOaLocation W16490674581 @default.
- W1649067458 hasConcept C104317684 @default.
- W1649067458 hasConcept C153911025 @default.
- W1649067458 hasConcept C159047783 @default.
- W1649067458 hasConcept C187882448 @default.
- W1649067458 hasConcept C190062978 @default.
- W1649067458 hasConcept C2522874641 @default.
- W1649067458 hasConcept C2779200571 @default.
- W1649067458 hasConcept C3675279 @default.
- W1649067458 hasConcept C55493867 @default.
- W1649067458 hasConcept C65666806 @default.
- W1649067458 hasConcept C67705224 @default.
- W1649067458 hasConcept C86803240 @default.
- W1649067458 hasConcept C88478588 @default.
- W1649067458 hasConceptScore W1649067458C104317684 @default.
- W1649067458 hasConceptScore W1649067458C153911025 @default.
- W1649067458 hasConceptScore W1649067458C159047783 @default.
- W1649067458 hasConceptScore W1649067458C187882448 @default.
- W1649067458 hasConceptScore W1649067458C190062978 @default.