FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1650376557> ?p ?o ?g. }

• W1650376557 endingPage "438" @default.
• W1650376557 startingPage "430" @default.
• W1650376557 abstract "We previously isolated three extracellular endogenous enzymes from a Streptomyces albogriseolus mutant strain which were targets of Streptomyces subtilisin inhibitor (SSI) (S. Taguchi, A. Odaka, Y. Watanabe, and H. Momose, Appl. Environ. Microbiol. 61:180-186, 1995). In the present study, of the three enzymes the largest one, with a molecular mass of 45 kDa (estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis), termed SAM-P45, has been characterized in detail. The entire gene encoding SAM-P45 was cloned as an approximately 10-kb fragment from S. albogriseolus S-3253 genomic DNA into an Escherichia coli host by using a shuttle plasmid vector. The amino acid sequence corresponding to the internal region of SAM-P45, deduced from the nucleotide sequence of the gene, revealed high homology, particularly in three regions around the active-site residues (Asp, His, and Ser), with the amino acid sequences of the mature domain of subtilisin-like serine proteases. In order to investigate the enzymatic properties of this protease, recombinant SAM-P45 was overproduced in Streptomyces coelicolor by using a strong SSI gene promoter. Sequence analysis of the SAM-P45 gene and peptide mapping of the purified SAM-P45 suggested that it is synthesized as a large precursor protein containing a large C-terminal prodomain (494 residues) in addition to an N-terminal preprodomain (23 and 172 residues). A high proportion of basic amino acids in the C-terminal prodomain was considered to serve an element interactive with the phospholipid bilayer existing in the C-terminal prodomain, as found in other membrane-anchoring proteases of gram-positive bacteria. It is noteworthy that SAM-P45 was found to prefer basic amino acids to aromatic or aliphatic amino acids in contrast to subtilisin BPN', which has a broad substrate specificity. The hydrolysis by SAM-P45 of the synthetic substrate (N-succinyl-L-Gly-L-Pro-L-Lys-p-nitroanilide) most preferred by this enzyme was inhibited by SSI, chymostatin, and EDTA. The proteolytic activity of SAM-P45 was stimulated by the divalent cations Ca2+ and Mg2+. From these findings, we conclude that SAM-P45 interacts with SSI and can be categorized as a novel member of the subtilisin-like serine protease family." @default.
• W1650376557 created "2016-06-24" @default.
• W1650376557 creator A5020653533 @default.
• W1650376557 creator A5022390623 @default.
• W1650376557 creator A5027215968 @default.
• W1650376557 creator A5028421346 @default.
• W1650376557 creator A5064090630 @default.
• W1650376557 creator A5065012187 @default.
• W1650376557 date "1997-01-01" @default.
• W1650376557 modified "2023-10-01" @default.
• W1650376557 title "A novel member of the subtilisin-like protease family from Streptomyces albogriseolus" @default.
• W1650376557 cites W1480056716 @default.
• W1650376557 cites W1506771283 @default.
• W1650376557 cites W1574840625 @default.
• W1650376557 cites W1580118319 @default.
• W1650376557 cites W1588598829 @default.
• W1650376557 cites W1601256941 @default.
• W1650376557 cites W1749453950 @default.
• W1650376557 cites W1765698972 @default.
• W1650376557 cites W1787509813 @default.
• W1650376557 cites W1826861965 @default.
• W1650376557 cites W1871935420 @default.
• W1650376557 cites W1964756553 @default.
• W1650376557 cites W1969756714 @default.
• W1650376557 cites W1970224014 @default.
• W1650376557 cites W1984247755 @default.
• W1650376557 cites W1991101546 @default.
• W1650376557 cites W2000729321 @default.
• W1650376557 cites W2004115138 @default.
• W1650376557 cites W2010994037 @default.
• W1650376557 cites W2015204736 @default.
• W1650376557 cites W2018289835 @default.
• W1650376557 cites W2018291258 @default.
• W1650376557 cites W2027118671 @default.
• W1650376557 cites W2040862545 @default.
• W1650376557 cites W2042096080 @default.
• W1650376557 cites W2043855562 @default.
• W1650376557 cites W2048930029 @default.
• W1650376557 cites W2062681653 @default.
• W1650376557 cites W2063487760 @default.
• W1650376557 cites W2067133968 @default.
• W1650376557 cites W2069394872 @default.
• W1650376557 cites W2083093015 @default.
• W1650376557 cites W2090784961 @default.
• W1650376557 cites W2091153733 @default.
• W1650376557 cites W2097184060 @default.
• W1650376557 cites W2100837269 @default.
• W1650376557 cites W2102552259 @default.
• W1650376557 cites W2112636022 @default.
• W1650376557 cites W2116638249 @default.
• W1650376557 cites W2138879032 @default.
• W1650376557 cites W2138913088 @default.
• W1650376557 cites W2141219803 @default.
• W1650376557 cites W2146180503 @default.
• W1650376557 cites W2151683156 @default.
• W1650376557 cites W2166240440 @default.
• W1650376557 cites W2173786024 @default.
• W1650376557 doi "https://doi.org/10.1128/jb.179.2.430-438.1997" @default.
• W1650376557 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/178713" @default.
• W1650376557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8990295" @default.
• W1650376557 hasPublicationYear "1997" @default.
• W1650376557 type Work @default.
• W1650376557 sameAs 1650376557 @default.
• W1650376557 citedByCount "27" @default.
• W1650376557 countsByYear W16503765572015 @default.
• W1650376557 countsByYear W16503765572016 @default.
• W1650376557 countsByYear W16503765572018 @default.
• W1650376557 countsByYear W16503765572020 @default.
• W1650376557 countsByYear W16503765572022 @default.
• W1650376557 crossrefType "journal-article" @default.
• W1650376557 hasAuthorship W1650376557A5020653533 @default.
• W1650376557 hasAuthorship W1650376557A5022390623 @default.
• W1650376557 hasAuthorship W1650376557A5027215968 @default.
• W1650376557 hasAuthorship W1650376557A5028421346 @default.
• W1650376557 hasAuthorship W1650376557A5064090630 @default.
• W1650376557 hasAuthorship W1650376557A5065012187 @default.
• W1650376557 hasBestOaLocation W16503765572 @default.
• W1650376557 hasConcept C104317684 @default.
• W1650376557 hasConcept C153911025 @default.
• W1650376557 hasConcept C167625842 @default.
• W1650376557 hasConcept C181199279 @default.
• W1650376557 hasConcept C182220744 @default.
• W1650376557 hasConcept C2776714187 @default.
• W1650376557 hasConcept C2777807008 @default.
• W1650376557 hasConcept C2779811187 @default.
• W1650376557 hasConcept C55493867 @default.
• W1650376557 hasConcept C84148353 @default.
• W1650376557 hasConcept C86803240 @default.
• W1650376557 hasConceptScore W1650376557C104317684 @default.
• W1650376557 hasConceptScore W1650376557C153911025 @default.
• W1650376557 hasConceptScore W1650376557C167625842 @default.
• W1650376557 hasConceptScore W1650376557C181199279 @default.
• W1650376557 hasConceptScore W1650376557C182220744 @default.
• W1650376557 hasConceptScore W1650376557C2776714187 @default.
• W1650376557 hasConceptScore W1650376557C2777807008 @default.
• W1650376557 hasConceptScore W1650376557C2779811187 @default.
• W1650376557 hasConceptScore W1650376557C55493867 @default.
• W1650376557 hasConceptScore W1650376557C84148353 @default.

• next