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- W1650603908 abstract "The autotransporter protein antigen 43 from Escherichia coli was recombinantly expressed in the cyanobacterium Synechocystis sp. PCC6803, under the regulation of the green light-inducible promoter PcpcG2. Antigen 43 expression was induced in response to green light, resulting in the constant accumulation of the protein for at least four days. The complete post-translational processing of the antigen 43 protein was confirmed by Western blot analysis. The resulting antigen 43 α chain was essentially all displayed on the cell surface, as confirmed by proteinase K treatment. Although microbial cell-surface-display technology has a wide range of biotechnological applications, this is the first report of an efficient method for the display of recombinant proteins in cyanobacteria." @default.
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- W1650603908 date "2015-11-01" @default.
- W1650603908 modified "2023-10-18" @default.
- W1650603908 title "Efficient surface-display of autotransporter proteins in cyanobacteria" @default.
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- W1650603908 doi "https://doi.org/10.1016/j.algal.2015.09.013" @default.
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