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• W1650720892 endingPage "2435" @default.
• W1650720892 startingPage "2423" @default.
• W1650720892 abstract "The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell in the absence of appropriate negative regulatory elements encoded by the korA, korB, korC, and korE determinants. We have proposed that the functions of the kil loci are related to RK2 maintenance or host range. Here, we report the nucleotide sequence of a 2.44-kb region that includes the lethal kilB determinant. We identified the first three genes of the kilB operon (designated klbA, klbB, and klbC), and we determined by deletion analysis that the host-lethal phenotype requires klbB. The predicted amino acid sequence of the 34,995-Da klbA product reveals a potential ATP-binding fold. The klbB product is predicted to be a membrane protein with a molecular mass of 15,012 Da with homology to the RK2 KlaC membrane protein encoded by the kilA operon. The amino acid sequence of the 12,085-Da klbC product contains a perfect match to the leucine zipper motif common to eukaryotic regulatory proteins. Primer extension analysis revealed unambiguously that transcription of the kilB operon begins 46 nucleotides upstream of klbA. No transcription was initiated from the sequence previously presumed by other investigators to be the kilB promoter. The abundance of kilB transcripts is reduced in the presence of KorB, consistent with the prediction that KorB acts at the level of transcription. A degenerate KorB-binding site that contains a perfect half-palindrome overlaps the kilB promoter, but this site is insufficient for regulation by KorB. The region containing a KorB-binding site located 183 bp upstream of the transcriptional start is required for regulation by KorB, indicating that KorB acts at a distance to regulate transcription of kilB. Our studies with the mutant plasmid pRP101, a transfer-defective derivative of the RK2-like plasmid RP4, demonstrated that the kilB operon includes the conjugal transfer and surface exclusion genes of the Tra2 region. Nucleotide sequence analysis revealed that the transposon Tn7 insertion in pRP101 is located in the klbC gene, and complementation analysis showed that this mutation has a strong polar effect on the expression of genes for conjugal transfer and surface exclusion located several kilobases downstream. A klbA mutant was constructed and found to be both transfer defective and complementable, thus, demonstrating a requirement was constructed and found to be both transfer defective and complementable, thus demonstrating a requirement for klbA product in plasmid transmissibility. These results have demonstrated a role for the kilB operon in conjugal transfer. The kil-kor regulon of RK2 is the only known example of plasmid-mediated coregulation of replication and transfer." @default.
• W1650720892 created "2016-06-24" @default.
• W1650720892 creator A5017598017 @default.
• W1650720892 creator A5030746815 @default.
• W1650720892 creator A5035375716 @default.
• W1650720892 creator A5047383224 @default.
• W1650720892 creator A5090122536 @default.
• W1650720892 date "1993-04-01" @default.
• W1650720892 modified "2023-10-14" @default.
• W1650720892 title "Structure, function, and regulation of the kilB locus of promiscuous plasmid RK2" @default.
• W1650720892 cites W1482496920 @default.
• W1650720892 cites W1496631662 @default.
• W1650720892 cites W1505193925 @default.
• W1650720892 cites W151628207 @default.
• W1650720892 cites W1534826438 @default.
• W1650720892 cites W1537514932 @default.
• W1650720892 cites W1541473088 @default.
• W1650720892 cites W1557612166 @default.
• W1650720892 cites W1570512758 @default.
• W1650720892 cites W1579268353 @default.
• W1650720892 cites W1583873696 @default.
• W1650720892 cites W1601687245 @default.
• W1650720892 cites W1715057838 @default.
• W1650720892 cites W1730089026 @default.
• W1650720892 cites W1791247791 @default.
• W1650720892 cites W1895988065 @default.
• W1650720892 cites W1902662884 @default.
• W1650720892 cites W1936065549 @default.
• W1650720892 cites W1947248835 @default.
• W1650720892 cites W1949766905 @default.
• W1650720892 cites W1956828098 @default.
• W1650720892 cites W1964152461 @default.
• W1650720892 cites W1964441761 @default.
• W1650720892 cites W1967164846 @default.
• W1650720892 cites W1970648090 @default.
• W1650720892 cites W1973091470 @default.
• W1650720892 cites W1973529320 @default.
• W1650720892 cites W1974985494 @default.
• W1650720892 cites W1976799792 @default.
• W1650720892 cites W1977482273 @default.
• W1650720892 cites W1985717726 @default.
• W1650720892 cites W1986510350 @default.
• W1650720892 cites W1988980407 @default.
• W1650720892 cites W1991536431 @default.
• W1650720892 cites W1994503800 @default.
• W1650720892 cites W1995122455 @default.
• W1650720892 cites W1999857608 @default.
• W1650720892 cites W2005393972 @default.
• W1650720892 cites W2006410528 @default.
• W1650720892 cites W2008570049 @default.
• W1650720892 cites W2009310436 @default.
• W1650720892 cites W2013718526 @default.
• W1650720892 cites W2014117083 @default.
• W1650720892 cites W2018181625 @default.
• W1650720892 cites W2018675618 @default.
• W1650720892 cites W2022129000 @default.
• W1650720892 cites W2027229485 @default.
• W1650720892 cites W2028622989 @default.
• W1650720892 cites W2031121939 @default.
• W1650720892 cites W2032118018 @default.
• W1650720892 cites W2032254782 @default.
• W1650720892 cites W2032776824 @default.
• W1650720892 cites W2035187003 @default.
• W1650720892 cites W2035985406 @default.
• W1650720892 cites W2038804719 @default.
• W1650720892 cites W2039672848 @default.
• W1650720892 cites W2043009008 @default.
• W1650720892 cites W2044155415 @default.
• W1650720892 cites W2044462665 @default.
• W1650720892 cites W2044952215 @default.
• W1650720892 cites W2050108888 @default.
• W1650720892 cites W2050325588 @default.
• W1650720892 cites W2050796343 @default.
• W1650720892 cites W2052503008 @default.
• W1650720892 cites W2053974374 @default.
• W1650720892 cites W2054595484 @default.
• W1650720892 cites W2058125728 @default.
• W1650720892 cites W2061700743 @default.
• W1650720892 cites W2064164532 @default.
• W1650720892 cites W2065824991 @default.
• W1650720892 cites W2068090287 @default.
• W1650720892 cites W2068419274 @default.
• W1650720892 cites W2071424950 @default.
• W1650720892 cites W2079672141 @default.
• W1650720892 cites W2082003231 @default.
• W1650720892 cites W2085290399 @default.
• W1650720892 cites W2086536186 @default.
• W1650720892 cites W2087945732 @default.
• W1650720892 cites W2096623504 @default.
• W1650720892 cites W2107089536 @default.
• W1650720892 cites W2111148495 @default.
• W1650720892 cites W2129328541 @default.
• W1650720892 cites W2132417094 @default.
• W1650720892 cites W2133335149 @default.
• W1650720892 cites W2138104394 @default.
• W1650720892 cites W2138270253 @default.
• W1650720892 cites W2140756446 @default.
• W1650720892 cites W2141653906 @default.

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