FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1650737533> ?p ?o ?g. }

• W1650737533 endingPage "4186" @default.
• W1650737533 startingPage "4178" @default.
• W1650737533 abstract "sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is synthesized from an inactive precursor protein (P31). The structural gene (sigE) for P31 was reengineered by oligonucleotide-directed mutagenesis to encode sigma E directly. The sequence specifying the first amino acid of sigma E (GGC) was placed immediately downstream of the initiating codon (ATG) of P31. The resulting sigE allele (sigE delta 84) encodes a sigma E-like protein which differs from the processed product by a single Met residue at its amino terminus. B. subtilis strains which carried this allele were Spo- and contained no detectable sigma E. The sigE delta 84 allele generated a product in Escherichia coli which, by quantitative Western immunoblot analysis, was present at 10 to 20% of the level of product (P31) obtained from a wild-type allele. A sigma E-like product was also not detected in two B. subtilis strains with missense mutations in the sequence encoding the processed region of P31. These results suggest that sigma E is a highly labile protein that is stabilized during its synthesis by an element of the precursor sequence. A mutant allele (sigE delta 48) which made an active sigma E-like protein in B. subtilis was isolated. This gene specified a product in which five amino acids, not derived from the P31 processed region, were joined to P31 at a position eight amino acids upstream of the processing site. The sigE delta 48 product was not processed, but it activated the sigma E -dependent spoIID promoter in vivo. The sigE delta 48 product therefore lost both an essential target for processing and a region which inhibited sigma sigma E activity. Cells which carried sig E delta 48 were Spo-. The basis of the sigE delta 48-dependent defect in sporulation is unknown, but the sigma E delta 48 activity appeared to persist beyond the time in development (4 h after onset sporulation) when wild-type sigma E activity declines. Thus, it may interfere with the proper regulation of late sporulation genes." @default.
• W1650737533 created "2016-06-24" @default.
• W1650737533 creator A5016899531 @default.
• W1650737533 creator A5047851402 @default.
• W1650737533 creator A5076508796 @default.
• W1650737533 date "1990-08-01" @default.
• W1650737533 modified "2023-09-26" @default.
• W1650737533 title "Phenotypes of Bacillus subtilis mutants altered in the precursor-specific region of sigma E" @default.
• W1650737533 cites W1519537394 @default.
• W1650737533 cites W1522024616 @default.
• W1650737533 cites W1523879423 @default.
• W1650737533 cites W1539601278 @default.
• W1650737533 cites W1552212939 @default.
• W1650737533 cites W1593097980 @default.
• W1650737533 cites W1897953158 @default.
• W1650737533 cites W1964462346 @default.
• W1650737533 cites W1964511689 @default.
• W1650737533 cites W1986642393 @default.
• W1650737533 cites W2006495190 @default.
• W1650737533 cites W2020334067 @default.
• W1650737533 cites W2028622989 @default.
• W1650737533 cites W2030535034 @default.
• W1650737533 cites W2036931150 @default.
• W1650737533 cites W2053149333 @default.
• W1650737533 cites W2071056869 @default.
• W1650737533 cites W2092747903 @default.
• W1650737533 cites W2102838603 @default.
• W1650737533 cites W2115024759 @default.
• W1650737533 cites W2117072345 @default.
• W1650737533 cites W2138270253 @default.
• W1650737533 cites W2138840910 @default.
• W1650737533 cites W2152165465 @default.
• W1650737533 cites W4296765338 @default.
• W1650737533 doi "https://doi.org/10.1128/jb.172.8.4178-4186.1990" @default.
• W1650737533 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/213240" @default.
• W1650737533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2115864" @default.
• W1650737533 hasPublicationYear "1990" @default.
• W1650737533 type Work @default.
• W1650737533 sameAs 1650737533 @default.
• W1650737533 citedByCount "24" @default.
• W1650737533 crossrefType "journal-article" @default.
• W1650737533 hasAuthorship W1650737533A5016899531 @default.
• W1650737533 hasAuthorship W1650737533A5047851402 @default.
• W1650737533 hasAuthorship W1650737533A5076508796 @default.
• W1650737533 hasBestOaLocation W16507375332 @default.
• W1650737533 hasConcept C101762097 @default.
• W1650737533 hasConcept C104317684 @default.
• W1650737533 hasConcept C121332964 @default.
• W1650737533 hasConcept C143065580 @default.
• W1650737533 hasConcept C150194340 @default.
• W1650737533 hasConcept C153911025 @default.
• W1650737533 hasConcept C195286587 @default.
• W1650737533 hasConcept C2777272437 @default.
• W1650737533 hasConcept C2778049214 @default.
• W1650737533 hasConcept C47319516 @default.
• W1650737533 hasConcept C523546767 @default.
• W1650737533 hasConcept C54355233 @default.
• W1650737533 hasConcept C62520636 @default.
• W1650737533 hasConcept C86803240 @default.
• W1650737533 hasConceptScore W1650737533C101762097 @default.
• W1650737533 hasConceptScore W1650737533C104317684 @default.
• W1650737533 hasConceptScore W1650737533C121332964 @default.
• W1650737533 hasConceptScore W1650737533C143065580 @default.
• W1650737533 hasConceptScore W1650737533C150194340 @default.
• W1650737533 hasConceptScore W1650737533C153911025 @default.
• W1650737533 hasConceptScore W1650737533C195286587 @default.
• W1650737533 hasConceptScore W1650737533C2777272437 @default.
• W1650737533 hasConceptScore W1650737533C2778049214 @default.
• W1650737533 hasConceptScore W1650737533C47319516 @default.
• W1650737533 hasConceptScore W1650737533C523546767 @default.
• W1650737533 hasConceptScore W1650737533C54355233 @default.
• W1650737533 hasConceptScore W1650737533C62520636 @default.
• W1650737533 hasConceptScore W1650737533C86803240 @default.
• W1650737533 hasIssue "8" @default.
• W1650737533 hasLocation W16507375331 @default.
• W1650737533 hasLocation W16507375332 @default.
• W1650737533 hasLocation W16507375333 @default.
• W1650737533 hasOpenAccess W1650737533 @default.
• W1650737533 hasPrimaryLocation W16507375331 @default.
• W1650737533 hasRelatedWork W1597078147 @default.
• W1650737533 hasRelatedWork W1964462346 @default.
• W1650737533 hasRelatedWork W1972379376 @default.
• W1650737533 hasRelatedWork W2088114354 @default.
• W1650737533 hasRelatedWork W2097789878 @default.
• W1650737533 hasRelatedWork W2105279467 @default.
• W1650737533 hasRelatedWork W2111311526 @default.
• W1650737533 hasRelatedWork W2119248810 @default.
• W1650737533 hasRelatedWork W2132346329 @default.
• W1650737533 hasRelatedWork W2137360360 @default.
• W1650737533 hasVolume "172" @default.
• W1650737533 isParatext "false" @default.
• W1650737533 isRetracted "false" @default.
• W1650737533 magId "1650737533" @default.
• W1650737533 workType "article" @default.