Matches in SemOpenAlex for { <https://semopenalex.org/work/W165210009> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W165210009 endingPage "103" @default.
- W165210009 startingPage "93" @default.
- W165210009 abstract "Identification of phosphoproteins or phosphopeptides as cancer biomarkers is an emerging field in phosphoproteomics. Owing to the low stoichiometric nature of protein phosphorylation, phosphoproteins or phosphopeptides must be enriched prior to downstream mass spectrometry analysis. Titanium dioxide (TiO2) has been prevalently used to enrich phosphopeptides from complex proteome samples due to its high affinity for phosphopeptides, and the method is straightforward. In this protocol, an offline phosphopeptide enrichment procedure using TiO2 columns is described. Peptides from a proteome lysate are loaded onto a TiO2 column in an acidic environment, followed by column washing with aqueous, organic, and ammonium glutamate (NH4Glu) buffers at acidic conditions. Phosphopeptides are eluted using an ammonia solution at high pH. Use of NH4Glu significantly reduces nonspecific bindings while a high recovery rate (84 %) of phosphopeptides is retained. The method is optimized for large-scale phosphoproteomic analysis and phosphoprotein biomarker discovery starting from sub-milligram or milligrams of proteome samples." @default.
- W165210009 created "2016-06-24" @default.
- W165210009 creator A5019010663 @default.
- W165210009 creator A5088688923 @default.
- W165210009 date "2013-01-01" @default.
- W165210009 modified "2023-09-27" @default.
- W165210009 title "Phosphopeptide Enrichment Using Offline Titanium Dioxide Columns for Phosphoproteomics" @default.
- W165210009 cites W1971541253 @default.
- W165210009 cites W1975563654 @default.
- W165210009 cites W1983836332 @default.
- W165210009 cites W2008683084 @default.
- W165210009 cites W2009881053 @default.
- W165210009 cites W2017128805 @default.
- W165210009 cites W2022976863 @default.
- W165210009 cites W2033860382 @default.
- W165210009 cites W2033889320 @default.
- W165210009 cites W2052141890 @default.
- W165210009 cites W2055152749 @default.
- W165210009 cites W2057419053 @default.
- W165210009 cites W2063186742 @default.
- W165210009 cites W2099255819 @default.
- W165210009 cites W2136179119 @default.
- W165210009 cites W2169359398 @default.
- W165210009 doi "https://doi.org/10.1007/978-1-62703-360-2_8" @default.
- W165210009 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8480155" @default.
- W165210009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23625397" @default.
- W165210009 hasPublicationYear "2013" @default.
- W165210009 type Work @default.
- W165210009 sameAs 165210009 @default.
- W165210009 citedByCount "9" @default.
- W165210009 countsByYear W1652100092014 @default.
- W165210009 countsByYear W1652100092015 @default.
- W165210009 countsByYear W1652100092018 @default.
- W165210009 countsByYear W1652100092020 @default.
- W165210009 countsByYear W1652100092021 @default.
- W165210009 countsByYear W1652100092022 @default.
- W165210009 countsByYear W1652100092023 @default.
- W165210009 crossrefType "book-chapter" @default.
- W165210009 hasAuthorship W165210009A5019010663 @default.
- W165210009 hasAuthorship W165210009A5088688923 @default.
- W165210009 hasBestOaLocation W1652100092 @default.
- W165210009 hasConcept C104397665 @default.
- W165210009 hasConcept C11960822 @default.
- W165210009 hasConcept C162356407 @default.
- W165210009 hasConcept C185592680 @default.
- W165210009 hasConcept C2777061661 @default.
- W165210009 hasConcept C2779933727 @default.
- W165210009 hasConcept C43617362 @default.
- W165210009 hasConcept C55493867 @default.
- W165210009 hasConcept C6675166 @default.
- W165210009 hasConcept C73826308 @default.
- W165210009 hasConcept C87325107 @default.
- W165210009 hasConcept C97029542 @default.
- W165210009 hasConceptScore W165210009C104397665 @default.
- W165210009 hasConceptScore W165210009C11960822 @default.
- W165210009 hasConceptScore W165210009C162356407 @default.
- W165210009 hasConceptScore W165210009C185592680 @default.
- W165210009 hasConceptScore W165210009C2777061661 @default.
- W165210009 hasConceptScore W165210009C2779933727 @default.
- W165210009 hasConceptScore W165210009C43617362 @default.
- W165210009 hasConceptScore W165210009C55493867 @default.
- W165210009 hasConceptScore W165210009C6675166 @default.
- W165210009 hasConceptScore W165210009C73826308 @default.
- W165210009 hasConceptScore W165210009C87325107 @default.
- W165210009 hasConceptScore W165210009C97029542 @default.
- W165210009 hasLocation W1652100091 @default.
- W165210009 hasLocation W1652100092 @default.
- W165210009 hasLocation W1652100093 @default.
- W165210009 hasLocation W1652100094 @default.
- W165210009 hasOpenAccess W165210009 @default.
- W165210009 hasPrimaryLocation W1652100091 @default.
- W165210009 hasRelatedWork W143420355 @default.
- W165210009 hasRelatedWork W2002642674 @default.
- W165210009 hasRelatedWork W2013999682 @default.
- W165210009 hasRelatedWork W2081150154 @default.
- W165210009 hasRelatedWork W2244617816 @default.
- W165210009 hasRelatedWork W2414409576 @default.
- W165210009 hasRelatedWork W2491326239 @default.
- W165210009 hasRelatedWork W3095586928 @default.
- W165210009 hasRelatedWork W3181115501 @default.
- W165210009 hasRelatedWork W4206199981 @default.
- W165210009 isParatext "false" @default.
- W165210009 isRetracted "false" @default.
- W165210009 magId "165210009" @default.
- W165210009 workType "book-chapter" @default.