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- W1652396989 abstract "This chapter discusses the synthesis of N-acylneuraminic (sialic) acid 9-phosphate synthetase. N-acetyl- or N-glycolyl-D-mannosamine 6-phosphate is condensed with phosphoenolpyruvate to yield N-acylneuraminic acid 9-phosphate. Enzymatic activity is measured by determining the rate of formation of the N-acylneuraminic acid 9-phosphate with a modification of the thiobarbituric acid colorimetric method of Aminoff. Each of the reagents used is adjusted to pH 7.8 with ammonium hydroxide immediately before use. Sialic acids are determined by a modification of the thiobarbituric acid method (TBA). Unless otherwise specified, all operations are conducted between 0° and 4°, buffers contain 1% 2-mercaptoethanol, and all centrifugations are performed at 35,000 g. Because of the relative instability of the enzyme preparation, the entire fractionation procedure is conducted as rapidly as possible. The quantity of solid ammonium sulfate used to obtain each fraction is expressed as the percentage of the quantity required to saturate the solution at 0°. Enzymatic activity is manifested only with N-acetyl- and N-glycolyl-D-mannosamine-6-P as substrates." @default.
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- W1652396989 date "1966-01-01" @default.
- W1652396989 modified "2023-09-27" @default.
- W1652396989 title "[29] N-acylneuraminic (sialic) acid 9-phosphate synthetase" @default.
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- W1652396989 doi "https://doi.org/10.1016/0076-6879(66)08033-9" @default.
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