Matches in SemOpenAlex for { <https://semopenalex.org/work/W1652890129> ?p ?o ?g. }
- W1652890129 abstract "Publisher Summary This chapter describes a method for fractionating chromatin that is based on specific hybridization between soluble chromatin fragments and oligonucleotides. This method, termed “nucleoprotein hybridization,” targets a specific gene on the basis of its DNA sequence. This technique makes it possible to isolate the chromatin of defined DNA sequence irrespective of its functional state. Alternative protocols for chromatin fractionation have employed differential solubility of chromatin fragments after mild nuclease digestion, immunoprecipitation, and mercury affinity columns. These alternative techniques yield a fraction enriched in active or inactive chromatin representing an ensemble of different genes. The chapter schematically represents seven steps of nucleoprotein hybridization. The feasibility of the technique has been proved with the SV40 model system. SV40 could be mixed with an excess of exogenous sea-urchin chromatin and then isolated to 88% purity corresponding to a 115-fold enrichment. The chapter also describes the first purification of active and inactive cellular genes as chromatin using the nucleoprotein hybridization. The Strongylocentrotus purpuratus sea urchin early histone gene repeat (SUEHGR) was chosen as a target for the first application of the technique." @default.
- W1652890129 created "2016-06-24" @default.
- W1652890129 creator A5016208809 @default.
- W1652890129 creator A5018037163 @default.
- W1652890129 creator A5030537443 @default.
- W1652890129 creator A5034432360 @default.
- W1652890129 creator A5055565815 @default.
- W1652890129 creator A5059681234 @default.
- W1652890129 date "1991-01-01" @default.
- W1652890129 modified "2023-10-16" @default.
- W1652890129 title "Chapter 13 The Nucleoprotein Hybridization Method for Isolating Active and Inactive Genes as Chromatin" @default.
- W1652890129 cites W123191937 @default.
- W1652890129 cites W1526949464 @default.
- W1652890129 cites W1530844112 @default.
- W1652890129 cites W1539547828 @default.
- W1652890129 cites W1549020945 @default.
- W1652890129 cites W1592136539 @default.
- W1652890129 cites W1740684741 @default.
- W1652890129 cites W1874007049 @default.
- W1652890129 cites W1913193070 @default.
- W1652890129 cites W1971314065 @default.
- W1652890129 cites W1976431835 @default.
- W1652890129 cites W1977402251 @default.
- W1652890129 cites W1978831455 @default.
- W1652890129 cites W1980795280 @default.
- W1652890129 cites W1986383947 @default.
- W1652890129 cites W1987799155 @default.
- W1652890129 cites W1987904181 @default.
- W1652890129 cites W1990659134 @default.
- W1652890129 cites W1993704650 @default.
- W1652890129 cites W1998485762 @default.
- W1652890129 cites W1999939543 @default.
- W1652890129 cites W2002651868 @default.
- W1652890129 cites W2004785999 @default.
- W1652890129 cites W2006043386 @default.
- W1652890129 cites W2006450491 @default.
- W1652890129 cites W2008143037 @default.
- W1652890129 cites W2011057633 @default.
- W1652890129 cites W2012096611 @default.
- W1652890129 cites W2015315939 @default.
- W1652890129 cites W2018307111 @default.
- W1652890129 cites W2020805433 @default.
- W1652890129 cites W2023928518 @default.
- W1652890129 cites W2028290956 @default.
- W1652890129 cites W2029125369 @default.
- W1652890129 cites W2030628228 @default.
- W1652890129 cites W2035276244 @default.
- W1652890129 cites W2042735758 @default.
- W1652890129 cites W2043420317 @default.
- W1652890129 cites W2044267903 @default.
- W1652890129 cites W2045951304 @default.
- W1652890129 cites W2049303884 @default.
- W1652890129 cites W2049869291 @default.
- W1652890129 cites W2057047962 @default.
- W1652890129 cites W2060917177 @default.
- W1652890129 cites W2063550395 @default.
- W1652890129 cites W2065634301 @default.
- W1652890129 cites W2067245659 @default.
- W1652890129 cites W2078825043 @default.
- W1652890129 cites W2079093064 @default.
- W1652890129 cites W2081832877 @default.
- W1652890129 cites W2086114437 @default.
- W1652890129 cites W2091752191 @default.
- W1652890129 cites W2092177846 @default.
- W1652890129 cites W2099114692 @default.
- W1652890129 cites W2125658528 @default.
- W1652890129 cites W2166174738 @default.
- W1652890129 cites W2167110107 @default.
- W1652890129 cites W2282054059 @default.
- W1652890129 cites W2296021359 @default.
- W1652890129 cites W2992741145 @default.
- W1652890129 doi "https://doi.org/10.1016/s0091-679x(08)60579-8" @default.
- W1652890129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1664029" @default.
- W1652890129 hasPublicationYear "1991" @default.
- W1652890129 type Work @default.
- W1652890129 sameAs 1652890129 @default.
- W1652890129 citedByCount "0" @default.
- W1652890129 crossrefType "book-chapter" @default.
- W1652890129 hasAuthorship W1652890129A5016208809 @default.
- W1652890129 hasAuthorship W1652890129A5018037163 @default.
- W1652890129 hasAuthorship W1652890129A5030537443 @default.
- W1652890129 hasAuthorship W1652890129A5034432360 @default.
- W1652890129 hasAuthorship W1652890129A5055565815 @default.
- W1652890129 hasAuthorship W1652890129A5059681234 @default.
- W1652890129 hasConcept C101762097 @default.
- W1652890129 hasConcept C104317684 @default.
- W1652890129 hasConcept C134320426 @default.
- W1652890129 hasConcept C150194340 @default.
- W1652890129 hasConcept C153911025 @default.
- W1652890129 hasConcept C176619506 @default.
- W1652890129 hasConcept C2777271071 @default.
- W1652890129 hasConcept C2777569064 @default.
- W1652890129 hasConcept C54355233 @default.
- W1652890129 hasConcept C552990157 @default.
- W1652890129 hasConcept C60325843 @default.
- W1652890129 hasConcept C64927066 @default.
- W1652890129 hasConcept C67258246 @default.
- W1652890129 hasConcept C83640560 @default.
- W1652890129 hasConcept C84772758 @default.
- W1652890129 hasConcept C86803240 @default.