Matches in SemOpenAlex for { <https://semopenalex.org/work/W1655025181> ?p ?o ?g. }
- W1655025181 endingPage "972" @default.
- W1655025181 startingPage "960" @default.
- W1655025181 abstract "Erwinia chrysanthemi causes soft-rot disease in a great variety of plants. In addition to the depolymerizing activity of plant cell wall-degrading enzymes, iron acquisition and resistance to oxidative stress contribute greatly to the virulence of this pathogen. Here, we studied the pin10 locus originally thought to encode new virulence factors. The sequence analysis revealed six open reading frames that were homologous to the Escherichia coli sufA, sufB, sufC, sufD, sufS and sufE genes. Sequence similarity searching predicted that (i) SufA, SufB, SufD, SufS and SufE proteins are involved in iron metabolism and possibly in Fe–S cluster assembly; and (ii) SufC is an ATPase of an ABC transporter. The reverse transcription–polymerase chain reaction procedure showed that the sufABCDSE genes constitute an operon. Expression of a sufB::uidA fusion was found to be induced in iron-deficient growth conditions and to be repressed by the iron-sensing Fur repressor. Each of the six suf genes was inactivated by the insertion of a cassette generating a non-polar mutation. The intracellular iron level in the sufA, sufB, sufC, sufS and sufE mutants was higher than in the wild type, as assessed by increased sensitivity to the iron-activated antibiotic streptonigrin. In addition, inactivation of sufC and sufD led to increased sensitivity to paraquat. Virulence tests showed that sufA and sufC mutants exhibited reduced ability to cause maceration of chicory leaves, whereas a functional sufC gene was necessary for the bacteria to cause systemic invasion of Saintpaulia ionantha. The E. coli sufC homologue was inactivated by reverse genetic. This mutation was found to modify the soxR-dependent induction of soxS gene expression. We discuss the possibility that SufC is a versatile ATPase that can associate either with the other Suf proteins to form a Fe–S cluster-assembling machinery or with membrane proteins encoded elsewhere in the chromosome to form an Fe–S ABC exporter. Overall, these results stress the importance of the connection between iron metabolism and oxidative stress during the early steps of infection by E. chrysanthemi." @default.
- W1655025181 created "2016-06-24" @default.
- W1655025181 creator A5014078114 @default.
- W1655025181 creator A5019487938 @default.
- W1655025181 creator A5035819625 @default.
- W1655025181 creator A5043621822 @default.
- W1655025181 creator A5059049734 @default.
- W1655025181 date "2001-12-21" @default.
- W1655025181 modified "2023-10-18" @default.
- W1655025181 title "SoxR-dependent response to oxidative stress and virulence of Erwinia chrysanthemi: the key role of SufC, an orphan ABC ATPase" @default.
- W1655025181 cites W1484313557 @default.
- W1655025181 cites W1532421291 @default.
- W1655025181 cites W1549958659 @default.
- W1655025181 cites W1578283411 @default.
- W1655025181 cites W1884874540 @default.
- W1655025181 cites W1960048856 @default.
- W1655025181 cites W1973318532 @default.
- W1655025181 cites W1981871034 @default.
- W1655025181 cites W1988994128 @default.
- W1655025181 cites W1994021560 @default.
- W1655025181 cites W2002390714 @default.
- W1655025181 cites W2003418336 @default.
- W1655025181 cites W2024152854 @default.
- W1655025181 cites W2028622989 @default.
- W1655025181 cites W2028890624 @default.
- W1655025181 cites W2030793312 @default.
- W1655025181 cites W2036122232 @default.
- W1655025181 cites W2041508675 @default.
- W1655025181 cites W2074321943 @default.
- W1655025181 cites W2085728679 @default.
- W1655025181 cites W2087921651 @default.
- W1655025181 cites W2088619559 @default.
- W1655025181 cites W2109162773 @default.
- W1655025181 cites W2121705881 @default.
- W1655025181 cites W2125480801 @default.
- W1655025181 cites W2130732935 @default.
- W1655025181 cites W2148147966 @default.
- W1655025181 cites W2160084442 @default.
- W1655025181 cites W2161767156 @default.
- W1655025181 cites W2162097287 @default.
- W1655025181 cites W2164149612 @default.
- W1655025181 cites W2164197559 @default.
- W1655025181 cites W2169927972 @default.
- W1655025181 cites W217428907 @default.
- W1655025181 cites W2405302058 @default.
- W1655025181 doi "https://doi.org/10.1046/j.1365-2958.2001.02288.x" @default.
- W1655025181 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11251816" @default.
- W1655025181 hasPublicationYear "2001" @default.
- W1655025181 type Work @default.
- W1655025181 sameAs 1655025181 @default.
- W1655025181 citedByCount "166" @default.
- W1655025181 countsByYear W16550251812012 @default.
- W1655025181 countsByYear W16550251812013 @default.
- W1655025181 countsByYear W16550251812014 @default.
- W1655025181 countsByYear W16550251812015 @default.
- W1655025181 countsByYear W16550251812016 @default.
- W1655025181 countsByYear W16550251812017 @default.
- W1655025181 countsByYear W16550251812018 @default.
- W1655025181 countsByYear W16550251812019 @default.
- W1655025181 countsByYear W16550251812020 @default.
- W1655025181 countsByYear W16550251812021 @default.
- W1655025181 countsByYear W16550251812022 @default.
- W1655025181 countsByYear W16550251812023 @default.
- W1655025181 crossrefType "journal-article" @default.
- W1655025181 hasAuthorship W1655025181A5014078114 @default.
- W1655025181 hasAuthorship W1655025181A5019487938 @default.
- W1655025181 hasAuthorship W1655025181A5035819625 @default.
- W1655025181 hasAuthorship W1655025181A5043621822 @default.
- W1655025181 hasAuthorship W1655025181A5059049734 @default.
- W1655025181 hasBestOaLocation W16550251811 @default.
- W1655025181 hasConcept C104317684 @default.
- W1655025181 hasConcept C141315368 @default.
- W1655025181 hasConcept C143065580 @default.
- W1655025181 hasConcept C149011108 @default.
- W1655025181 hasConcept C181199279 @default.
- W1655025181 hasConcept C203075996 @default.
- W1655025181 hasConcept C23265538 @default.
- W1655025181 hasConcept C44312359 @default.
- W1655025181 hasConcept C54355233 @default.
- W1655025181 hasConcept C547475151 @default.
- W1655025181 hasConcept C55493867 @default.
- W1655025181 hasConcept C60987743 @default.
- W1655025181 hasConcept C75578959 @default.
- W1655025181 hasConcept C86803240 @default.
- W1655025181 hasConcept C89423630 @default.
- W1655025181 hasConceptScore W1655025181C104317684 @default.
- W1655025181 hasConceptScore W1655025181C141315368 @default.
- W1655025181 hasConceptScore W1655025181C143065580 @default.
- W1655025181 hasConceptScore W1655025181C149011108 @default.
- W1655025181 hasConceptScore W1655025181C181199279 @default.
- W1655025181 hasConceptScore W1655025181C203075996 @default.
- W1655025181 hasConceptScore W1655025181C23265538 @default.
- W1655025181 hasConceptScore W1655025181C44312359 @default.
- W1655025181 hasConceptScore W1655025181C54355233 @default.
- W1655025181 hasConceptScore W1655025181C547475151 @default.
- W1655025181 hasConceptScore W1655025181C55493867 @default.
- W1655025181 hasConceptScore W1655025181C60987743 @default.
- W1655025181 hasConceptScore W1655025181C75578959 @default.