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- W1655580624 abstract "Porcine amniotic fluid-derived stem(AFS) cells were isolated from amniotic fluid of 30-day porcine fetuses.Plasmid containing enhanced green fluorescence protein gene(EGFP) was transfected into AFS cells by lipofection.EGFP gene transfected AFS cells were induced to differentiate into cell types of osteogenic and myogenic lineages.Expanded AFS cells and differentiated cells were harvested for analysis of markers using reverse transcription-polymerase chain reaction(RT-PCR).The results showed that the positive AFS cells were obtained.AFS cells expressed octamer transcription factor 4(Oct4), CD-90 and Sox2.The EGFP gene transfected AFS cells were differentiated into osteoblast(osteonectin+ and osteocalcin+) and myocyte(myf-6+ and myoD+).The results demonstrated that AFS cells were easily isolated and cultured from amniotic fluid of porcine fetuses, the EGFP gene transfected AFS cells were capable of self-renewal, and broadly multipotent.EGFP can be used as a marker in monitoring AFS cells." @default.
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- W1655580624 date "2009-01-01" @default.
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- W1655580624 title "In vitro differentiation of enhanced green fluorescence protein gene (EGFP) transfected porcine amniotic fluid-derived stem cells." @default.
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