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- W1655996970 abstract "The aim of this work was to evaluate the effect of HECM-9 and TCM-199 as maturation media on maturation (MR), in vitro fertilization (IVF) and embryo development (ED) rate of oocytes from hair sheep collected from slaughter house ovaries. Cumulus-oocyte-complexes (COC) were obtained by manual aspiration, from 2-6mm diameter follicles. Groups of 10-20 COC, quality 1 and 2 were placed into 450μL of HECM-9 or TCM-199 and incubated 24h at 38.5 oC and 5% CO 2 . For IVF, COC were transferred to 450μL of SOFm+Oaa, 0.5 x 10 6 motile spermatozoa were added and then incubated at 38.5 oC and 5% CO 2. Alleged zygotes were transferred to 450μL of SOFm+Oaa+glucose. Embryo development and morphology were evaluated at 2, 4, 5 and 6 days of culture, not developed zygotes were removed on day 2 and the final rate of embryo production was determined on day 8 of culture. Oocyte MR showed no significant differences (P>0.05) between treatments (73.3 vs . 71.4 % HECM-9 and TCM-199, respectively). Fertilization rate was different (P vs. 28.6%, respectively). Embryo development was greater (P vs. 7.9%, respectively). We conclude that HECM-9 increased in vitro fertilization rate and embryo development in ovine oocytes." @default.
- W1655996970 created "2016-06-24" @default.
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- W1655996970 date "2008-12-01" @default.
- W1655996970 modified "2023-09-27" @default.
- W1655996970 title "EVALUATION OF TWO in vitro MATURATION MEDIUM FOR EMBRYO PRODUCTION IN SHEEP" @default.
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