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- W1657182270 abstract "A genetic screen was used to identify amino acid substitutions that enable the FokI restriction endonuclease to cleave DNA in cells that express the cognate methyltransferase activity. Missense mutations that give rise to this phenotype were isolated at eight different positions (G188K, P196S, T343I, S388N, S395F, E407K, E410K, D421N), clustered in two regions of the polypeptide sequence of FokI. Two of the mutant endonucleases (P196S and D421N) were purified to homogeneity and analyzed in detail. Both mutants cleave FokI target sites (5'-GGATG-3') in a manner similar to the wild-type enzyme. Neither mutant cleaved noncanonical sequences, but both efficiently cleaved DNA substrates containing hemi-methylated FokI sites. This class of mutations has not been observed with other restriction enzymes." @default.
- W1657182270 created "2016-06-24" @default.
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- W1657182270 date "1994-04-01" @default.
- W1657182270 modified "2023-09-30" @default.
- W1657182270 title "A novel class of FokI restriction endonuclease mutants that cleave hemi-methylated substrates." @default.
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- W1657182270 doi "https://doi.org/10.1016/s0021-9258(17)32715-1" @default.
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