FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1657353485> ?p ?o ?g. }

• W1657353485 endingPage "659" @default.
• W1657353485 startingPage "652" @default.
• W1657353485 abstract "Electron spin resonance (ESR) studies on spin trapping of superoxide and hydroxyl radicals by 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) were performed in NADPH-cytochrome P-450 reductase-paraquat systems at pH 7.4. Spin adduct concentrations were determined by comparing ESR spectra of the adducts with the ESR spectrum of a stable radical solution. Kinetic analysis in the presence of 100 microM desferrioxamine B (deferoxamine) showed that: 1) the oxidation of 1 mol of NADPH produces 2 mol of superoxide ions, all of which can be trapped by DMPO when extrapolated to infinite concentration; 2) the rate constant for the reaction of superoxide with DMPO was 1.2 M-1 s-1; 3) the superoxide spin adduct of DMPO (DMPO-OOH) decays with a half-life of 66 s and the maximum level of DMPO-OOH formed can be calculated by a simple steady state equation; and 4) 2.8% or less of the DMPO-OOH decay occurs through a reaction producing hydroxyl radicals. In the presence of 100 microM EDTA, 5 microM Fe(III) ions nearly completely inhibited the formation of the hydroxyl radical adduct of DMPO (DMPO-OH) as well as the formation of DMPO-OOH and, when 100 microM hydrogen peroxide was present, produced DMPO-OH exclusively. Fe(III)-EDTA is reduced by superoxide and the competition of superoxide and hydrogen peroxide in the reaction with Fe(II)-EDTA seems to be reflected in the amounts of DMPO-OOH and DMPO-OH detected. These effects of EDTA can be explained from known kinetic data including a rate constant of 6 x 10(4) M-1 s-1 for reduction of DMPO-OOH by Fe(II)-EDTA. The effect of diethylenetriamine pentaacetic acid (DETAPAC) on the formation of DMPO-OOH and DMPO-OH was between deferoxamine and EDTA, and about the same as that of endogenous chelator (phosphate)." @default.
• W1657353485 created "2016-06-24" @default.
• W1657353485 creator A5006739724 @default.
• W1657353485 creator A5025781966 @default.
• W1657353485 creator A5071524324 @default.
• W1657353485 date "1990-01-01" @default.
• W1657353485 modified "2023-09-30" @default.
• W1657353485 title "Kinetic studies on spin trapping of superoxide and hydroxyl radicals generated in NADPH-cytochrome P-450 reductase-paraquat systems. Effect of iron chelates." @default.
• W1657353485 cites W1479833941 @default.
• W1657353485 cites W1508450059 @default.
• W1657353485 cites W1510966084 @default.
• W1657353485 cites W1546482094 @default.
• W1657353485 cites W1547982266 @default.
• W1657353485 cites W1549222475 @default.
• W1657353485 cites W1553328291 @default.
• W1657353485 cites W1558162435 @default.
• W1657353485 cites W1563548293 @default.
• W1657353485 cites W1570089337 @default.
• W1657353485 cites W1595733202 @default.
• W1657353485 cites W1602276313 @default.
• W1657353485 cites W1825467595 @default.
• W1657353485 cites W1834755341 @default.
• W1657353485 cites W1937896568 @default.
• W1657353485 cites W1940436875 @default.
• W1657353485 cites W1955274404 @default.
• W1657353485 cites W1964162589 @default.
• W1657353485 cites W1966423929 @default.
• W1657353485 cites W1966644042 @default.
• W1657353485 cites W1970010697 @default.
• W1657353485 cites W1975114702 @default.
• W1657353485 cites W1983576601 @default.
• W1657353485 cites W1984583542 @default.
• W1657353485 cites W1985348295 @default.
• W1657353485 cites W1989813911 @default.
• W1657353485 cites W1991277063 @default.
• W1657353485 cites W1993257224 @default.
• W1657353485 cites W1995340760 @default.
• W1657353485 cites W1996236557 @default.
• W1657353485 cites W1997658949 @default.
• W1657353485 cites W2001834244 @default.
• W1657353485 cites W2002769318 @default.
• W1657353485 cites W2003842171 @default.
• W1657353485 cites W2008240103 @default.
• W1657353485 cites W2008738285 @default.
• W1657353485 cites W2010237899 @default.
• W1657353485 cites W2017015171 @default.
• W1657353485 cites W2021973381 @default.
• W1657353485 cites W2022472966 @default.
• W1657353485 cites W2024921655 @default.
• W1657353485 cites W2027744481 @default.
• W1657353485 cites W2033082907 @default.
• W1657353485 cites W2036829350 @default.
• W1657353485 cites W2037877887 @default.
• W1657353485 cites W2040660829 @default.
• W1657353485 cites W2040870649 @default.
• W1657353485 cites W2045120677 @default.
• W1657353485 cites W2045665780 @default.
• W1657353485 cites W2050994988 @default.
• W1657353485 cites W2054449926 @default.
• W1657353485 cites W2058689969 @default.
• W1657353485 cites W2064450459 @default.
• W1657353485 cites W2067717733 @default.
• W1657353485 cites W2072813924 @default.
• W1657353485 cites W2073482225 @default.
• W1657353485 cites W2074365079 @default.
• W1657353485 cites W2078100316 @default.
• W1657353485 cites W2082760425 @default.
• W1657353485 cites W2085276728 @default.
• W1657353485 cites W2087527475 @default.
• W1657353485 cites W2093550919 @default.
• W1657353485 cites W2094539803 @default.
• W1657353485 cites W2126370064 @default.
• W1657353485 cites W2165326144 @default.
• W1657353485 cites W2168207032 @default.
• W1657353485 cites W2270686920 @default.
• W1657353485 cites W2345447808 @default.
• W1657353485 cites W4235887790 @default.
• W1657353485 cites W96661271 @default.
• W1657353485 doi "https://doi.org/10.1016/s0021-9258(19)40099-9" @default.
• W1657353485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2153108" @default.
• W1657353485 hasPublicationYear "1990" @default.
• W1657353485 type Work @default.
• W1657353485 sameAs 1657353485 @default.
• W1657353485 citedByCount "61" @default.
• W1657353485 countsByYear W16573534852013 @default.
• W1657353485 countsByYear W16573534852016 @default.
• W1657353485 countsByYear W16573534852017 @default.
• W1657353485 countsByYear W16573534852018 @default.
• W1657353485 countsByYear W16573534852021 @default.
• W1657353485 crossrefType "journal-article" @default.
• W1657353485 hasAuthorship W1657353485A5006739724 @default.
• W1657353485 hasAuthorship W1657353485A5025781966 @default.
• W1657353485 hasAuthorship W1657353485A5071524324 @default.
• W1657353485 hasBestOaLocation W16573534851 @default.
• W1657353485 hasConcept C134651460 @default.
• W1657353485 hasConcept C139066938 @default.
• W1657353485 hasConcept C179104552 @default.
• W1657353485 hasConcept C181199279 @default.

• next