Matches in SemOpenAlex for { <https://semopenalex.org/work/W1657550065> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1657550065 endingPage "1304" @default.
- W1657550065 startingPage "1298" @default.
- W1657550065 abstract "Abstract Cholesterol homeostasis is maintained by coordinate regulation of endogenous synthesis and exogenous uptake of lipoprotein cholesterol by low density lipoprotein (LDL) receptors. In the lymphocyte, limiting the availability of exogenous cholesterol is known to increase the rate of endogenous sterol biosynthesis. However, the effect of cholesterol deprivation on the expression and regulation of the LDL receptor gene has not been delineated in lymphocytes. Here, LDL receptor mRNA was detected in freshly isolated human peripheral mononuclear cells. LDL receptor mRNA levels increased by 3-fold during a one-h in vitro culture in lipoprotein-deficient medium and by 6-fold during a 2-h incubation. Actinomycin D blocked the synthesis of LDL receptor mRNA in these cultures. However, neither cycloheximide nor LDL or oxygenated sterols suppressed the increase in LDL receptor mRNA levels observed after a 2-h incubation. The increase in LDL receptor mRNA was maintained for 24 h of culture in the absence of LDL. Ongoing gene transcription and not mRNA stabilization accounted for this expression. Inhibition of protein synthesis with cycloheximide completely prevented the sustained increase in LDL receptor mRNA levels measured after 24 h. Low concentrations of LDL (5 micrograms of cholesterol/ml) and oxygenated sterols also suppressed the level of LDL receptor mRNA measured after a 24-h incubation. These data show that the initial upregulation of LDL receptor gene expression is independent of protein synthesis and not suppressed by either LDL or oxygenated sterols. In contrast, the continued transcription necessary for the maintenance of steady-state levels of LDL receptor mRNA requires synthesis of new protein and is regulated by LDL and oxygenated sterols." @default.
- W1657550065 created "2016-06-24" @default.
- W1657550065 creator A5007342242 @default.
- W1657550065 creator A5043874873 @default.
- W1657550065 creator A5047065106 @default.
- W1657550065 date "1989-01-01" @default.
- W1657550065 modified "2023-09-29" @default.
- W1657550065 title "Regulation of Low Density Lipoprotein Receptor Gene Expression in Human Lymphocytes" @default.
- W1657550065 cites W1549703942 @default.
- W1657550065 cites W1568755186 @default.
- W1657550065 cites W1601687245 @default.
- W1657550065 cites W1631424226 @default.
- W1657550065 cites W1902852842 @default.
- W1657550065 cites W1967004332 @default.
- W1657550065 cites W1972968384 @default.
- W1657550065 cites W1995032745 @default.
- W1657550065 cites W1996241166 @default.
- W1657550065 cites W2001429874 @default.
- W1657550065 cites W2028683797 @default.
- W1657550065 cites W2048578125 @default.
- W1657550065 cites W2054073262 @default.
- W1657550065 cites W2088132089 @default.
- W1657550065 cites W2089126365 @default.
- W1657550065 cites W2092602709 @default.
- W1657550065 cites W2156799866 @default.
- W1657550065 cites W2184891169 @default.
- W1657550065 cites W2336778665 @default.
- W1657550065 cites W2576734941 @default.
- W1657550065 cites W4241531133 @default.
- W1657550065 doi "https://doi.org/10.1016/s0021-9258(19)85085-8" @default.
- W1657550065 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2910855" @default.
- W1657550065 hasPublicationYear "1989" @default.
- W1657550065 type Work @default.
- W1657550065 sameAs 1657550065 @default.
- W1657550065 citedByCount "40" @default.
- W1657550065 countsByYear W16575500652014 @default.
- W1657550065 countsByYear W16575500652015 @default.
- W1657550065 countsByYear W16575500652017 @default.
- W1657550065 countsByYear W16575500652018 @default.
- W1657550065 countsByYear W16575500652021 @default.
- W1657550065 crossrefType "journal-article" @default.
- W1657550065 hasAuthorship W1657550065A5007342242 @default.
- W1657550065 hasAuthorship W1657550065A5043874873 @default.
- W1657550065 hasAuthorship W1657550065A5047065106 @default.
- W1657550065 hasBestOaLocation W16575500651 @default.
- W1657550065 hasConcept C104317684 @default.
- W1657550065 hasConcept C134018914 @default.
- W1657550065 hasConcept C150194340 @default.
- W1657550065 hasConcept C153911025 @default.
- W1657550065 hasConcept C170493617 @default.
- W1657550065 hasConcept C2778163477 @default.
- W1657550065 hasConcept C2780072125 @default.
- W1657550065 hasConcept C43554185 @default.
- W1657550065 hasConcept C54355233 @default.
- W1657550065 hasConcept C6916431 @default.
- W1657550065 hasConcept C86803240 @default.
- W1657550065 hasConcept C95444343 @default.
- W1657550065 hasConceptScore W1657550065C104317684 @default.
- W1657550065 hasConceptScore W1657550065C134018914 @default.
- W1657550065 hasConceptScore W1657550065C150194340 @default.
- W1657550065 hasConceptScore W1657550065C153911025 @default.
- W1657550065 hasConceptScore W1657550065C170493617 @default.
- W1657550065 hasConceptScore W1657550065C2778163477 @default.
- W1657550065 hasConceptScore W1657550065C2780072125 @default.
- W1657550065 hasConceptScore W1657550065C43554185 @default.
- W1657550065 hasConceptScore W1657550065C54355233 @default.
- W1657550065 hasConceptScore W1657550065C6916431 @default.
- W1657550065 hasConceptScore W1657550065C86803240 @default.
- W1657550065 hasConceptScore W1657550065C95444343 @default.
- W1657550065 hasIssue "2" @default.
- W1657550065 hasLocation W16575500651 @default.
- W1657550065 hasOpenAccess W1657550065 @default.
- W1657550065 hasPrimaryLocation W16575500651 @default.
- W1657550065 hasRelatedWork W1812963099 @default.
- W1657550065 hasRelatedWork W1987308748 @default.
- W1657550065 hasRelatedWork W2009966535 @default.
- W1657550065 hasRelatedWork W2035816658 @default.
- W1657550065 hasRelatedWork W2038054558 @default.
- W1657550065 hasRelatedWork W2078923563 @default.
- W1657550065 hasRelatedWork W2093550999 @default.
- W1657550065 hasRelatedWork W2265598874 @default.
- W1657550065 hasRelatedWork W2345775285 @default.
- W1657550065 hasRelatedWork W2409468117 @default.
- W1657550065 hasVolume "264" @default.
- W1657550065 isParatext "false" @default.
- W1657550065 isRetracted "false" @default.
- W1657550065 magId "1657550065" @default.
- W1657550065 workType "article" @default.