FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1657881138> ?p ?o ?g. }

• W1657881138 endingPage "6682" @default.
• W1657881138 startingPage "6676" @default.
• W1657881138 abstract "In a previous publication (Narhi, L. O., and Fulco, A. J. (1986) J. Biol. Chem. 261, 7160-7169) we described the characterization of a 119,000-dalton P-450 cytochrome that is strongly induced by barbiturates in Bacillus megaterium. In the presence of NADPH and O2, this single polypeptide can catalyze the hydroxylation of long-chain fatty acids without the aid of any other protein. The gene encoding this unique monooxygenase (cytochrome P-450BM-3) has now been cloned by an immunochemical screening technique. The Escherichia coli clone harboring the recombinant plasmid produces a 119,000-dalton protein that appears to be electrophoretically and immunochemically identical to the B. megaterium enzyme and contains the same N-terminal amino acid sequence. The recombinant DNA product also exhibits the characteristic cytochrome P-450 spectrum and is fully functional as a fatty acid monooxygenase. In E. coli, the synthesis of P-450BM-3 is directed by its own promoter included in the DNA insert and proceeds constitutively at a very high rate but is not stimulated by pentobarbital. However, when the cloned P-450BM-3 gene, either intact or in a truncated form, is introduced back into B. megaterium via an E. coli/Bacillus subtilis shuttle vector, its expression is constitutively repressed but is induced by pentobarbital. This finding demonstrates that the regulatory region of the P-450BM-3 gene that responds to barbiturates is included in the cloned DNA. The evidence also indicates that pentobarbital cannot directly act on the gene to cause induction but presumably interacts with another component such as a repressor molecule that is present in B. megaterium but is absent in the E. coli clone." @default.
• W1657881138 created "2016-06-24" @default.
• W1657881138 creator A5003397600 @default.
• W1657881138 creator A5033595397 @default.
• W1657881138 date "1987-05-01" @default.
• W1657881138 modified "2023-09-30" @default.
• W1657881138 title "Cloning of the gene encoding a catalytically self-sufficient cytochrome P-450 fatty acid monooxygenase induced by barbiturates in Bacillus megaterium and its functional expression and regulation in heterologous (Escherichia coli) and homologous (Bacillus megaterium) hosts." @default.
• W1657881138 cites W1494980574 @default.
• W1657881138 cites W1497083865 @default.
• W1657881138 cites W1506043065 @default.
• W1657881138 cites W1512156562 @default.
• W1657881138 cites W1515424959 @default.
• W1657881138 cites W1838414788 @default.
• W1657881138 cites W1967565963 @default.
• W1657881138 cites W1970561598 @default.
• W1657881138 cites W1979665733 @default.
• W1657881138 cites W1992493201 @default.
• W1657881138 cites W1995429833 @default.
• W1657881138 cites W2019410656 @default.
• W1657881138 cites W2037256933 @default.
• W1657881138 cites W2045081422 @default.
• W1657881138 cites W2059913491 @default.
• W1657881138 cites W2080077801 @default.
• W1657881138 cites W2083705742 @default.
• W1657881138 cites W2090069414 @default.
• W1657881138 cites W2094439416 @default.
• W1657881138 cites W2100837269 @default.
• W1657881138 cites W2101108802 @default.
• W1657881138 cites W2128145915 @default.
• W1657881138 cites W2156508676 @default.
• W1657881138 cites W2161524167 @default.
• W1657881138 cites W2166260496 @default.
• W1657881138 cites W2178409011 @default.
• W1657881138 cites W51481324 @default.
• W1657881138 cites W949740512 @default.
• W1657881138 doi "https://doi.org/10.1016/s0021-9258(18)48295-6" @default.
• W1657881138 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3106359" @default.
• W1657881138 hasPublicationYear "1987" @default.
• W1657881138 type Work @default.
• W1657881138 sameAs 1657881138 @default.
• W1657881138 citedByCount "101" @default.
• W1657881138 countsByYear W16578811382012 @default.
• W1657881138 countsByYear W16578811382013 @default.
• W1657881138 countsByYear W16578811382014 @default.
• W1657881138 countsByYear W16578811382015 @default.
• W1657881138 countsByYear W16578811382016 @default.
• W1657881138 countsByYear W16578811382017 @default.
• W1657881138 countsByYear W16578811382018 @default.
• W1657881138 countsByYear W16578811382019 @default.
• W1657881138 countsByYear W16578811382020 @default.
• W1657881138 countsByYear W16578811382021 @default.
• W1657881138 countsByYear W16578811382022 @default.
• W1657881138 countsByYear W16578811382023 @default.
• W1657881138 crossrefType "journal-article" @default.
• W1657881138 hasAuthorship W1657881138A5003397600 @default.
• W1657881138 hasAuthorship W1657881138A5033595397 @default.
• W1657881138 hasBestOaLocation W16578811381 @default.
• W1657881138 hasConcept C104317684 @default.
• W1657881138 hasConcept C108305142 @default.
• W1657881138 hasConcept C121050878 @default.
• W1657881138 hasConcept C13002179 @default.
• W1657881138 hasConcept C150194340 @default.
• W1657881138 hasConcept C170344550 @default.
• W1657881138 hasConcept C181199279 @default.
• W1657881138 hasConcept C185592680 @default.
• W1657881138 hasConcept C19924922 @default.
• W1657881138 hasConcept C199360897 @default.
• W1657881138 hasConcept C2780115692 @default.
• W1657881138 hasConcept C2780768313 @default.
• W1657881138 hasConcept C2780989563 @default.
• W1657881138 hasConcept C40767141 @default.
• W1657881138 hasConcept C41008148 @default.
• W1657881138 hasConcept C523546767 @default.
• W1657881138 hasConcept C526171541 @default.
• W1657881138 hasConcept C54355233 @default.
• W1657881138 hasConcept C547475151 @default.
• W1657881138 hasConcept C55493867 @default.
• W1657881138 hasConcept C62231903 @default.
• W1657881138 hasConcept C86803240 @default.
• W1657881138 hasConceptScore W1657881138C104317684 @default.
• W1657881138 hasConceptScore W1657881138C108305142 @default.
• W1657881138 hasConceptScore W1657881138C121050878 @default.
• W1657881138 hasConceptScore W1657881138C13002179 @default.
• W1657881138 hasConceptScore W1657881138C150194340 @default.
• W1657881138 hasConceptScore W1657881138C170344550 @default.
• W1657881138 hasConceptScore W1657881138C181199279 @default.
• W1657881138 hasConceptScore W1657881138C185592680 @default.
• W1657881138 hasConceptScore W1657881138C19924922 @default.
• W1657881138 hasConceptScore W1657881138C199360897 @default.
• W1657881138 hasConceptScore W1657881138C2780115692 @default.
• W1657881138 hasConceptScore W1657881138C2780768313 @default.
• W1657881138 hasConceptScore W1657881138C2780989563 @default.
• W1657881138 hasConceptScore W1657881138C40767141 @default.
• W1657881138 hasConceptScore W1657881138C41008148 @default.
• W1657881138 hasConceptScore W1657881138C523546767 @default.
• W1657881138 hasConceptScore W1657881138C526171541 @default.
• W1657881138 hasConceptScore W1657881138C54355233 @default.
• W1657881138 hasConceptScore W1657881138C547475151 @default.

• next