Matches in SemOpenAlex for { <https://semopenalex.org/work/W1658011747> ?p ?o ?g. }
- W1658011747 endingPage "1988" @default.
- W1658011747 startingPage "1977" @default.
- W1658011747 abstract "We have defined the molecular basis for association of the PH domain of the Arf GAP ASAP1 with phospholipid bilayers. Structures of the unliganded and dibutyryl PtdIns(4,5)P2-bound PH domain were solved. PtdIns(4,5)P2 made contact with both a canonical site (C site) and an atypical site (A site). We hypothesized cooperative binding of PtdIns(4,5)P2 to the C site and a nonspecific anionic phospholipid to the A site. PtdIns(4,5)P2 dependence of binding to large unilamellar vesicles and GAP activity was sigmoidal, consistent with cooperative sites. In contrast, PtdIns(4,5)P2 binding to the PH domain of PLC δ1 was hyperbolic. Mutation of amino acids in either the C or A site resulted in decreased PtdIns(4,5)P2-dependent binding to vesicles and decreased GAP activity. The results support the idea of cooperative phospholipid binding to the C and A sites of the PH domain of ASAP1. We propose that the mechanism underlies rapid switching between active and inactive ASAP1." @default.
- W1658011747 created "2016-06-24" @default.
- W1658011747 creator A5002229441 @default.
- W1658011747 creator A5008383835 @default.
- W1658011747 creator A5009326851 @default.
- W1658011747 creator A5020500579 @default.
- W1658011747 creator A5023153444 @default.
- W1658011747 creator A5023309436 @default.
- W1658011747 creator A5024593024 @default.
- W1658011747 creator A5052840181 @default.
- W1658011747 creator A5061371289 @default.
- W1658011747 creator A5065084938 @default.
- W1658011747 creator A5076063652 @default.
- W1658011747 creator A5087158145 @default.
- W1658011747 date "2015-11-01" @default.
- W1658011747 modified "2023-10-10" @default.
- W1658011747 title "Molecular Basis for Cooperative Binding of Anionic Phospholipids to the PH Domain of the Arf GAP ASAP1" @default.
- W1658011747 cites W1501993347 @default.
- W1658011747 cites W1533618603 @default.
- W1658011747 cites W1539796472 @default.
- W1658011747 cites W1549031993 @default.
- W1658011747 cites W1567005075 @default.
- W1658011747 cites W1608221212 @default.
- W1658011747 cites W1964027175 @default.
- W1658011747 cites W1969134590 @default.
- W1658011747 cites W1969359875 @default.
- W1658011747 cites W1972762719 @default.
- W1658011747 cites W1979742234 @default.
- W1658011747 cites W1985510880 @default.
- W1658011747 cites W1988846133 @default.
- W1658011747 cites W1997784032 @default.
- W1658011747 cites W1999226878 @default.
- W1658011747 cites W2001641653 @default.
- W1658011747 cites W2009468127 @default.
- W1658011747 cites W2021597866 @default.
- W1658011747 cites W2022907895 @default.
- W1658011747 cites W2024700991 @default.
- W1658011747 cites W2027371255 @default.
- W1658011747 cites W2029354255 @default.
- W1658011747 cites W2030732654 @default.
- W1658011747 cites W2035713281 @default.
- W1658011747 cites W2039708677 @default.
- W1658011747 cites W2043312574 @default.
- W1658011747 cites W2060224232 @default.
- W1658011747 cites W2064971479 @default.
- W1658011747 cites W2065057589 @default.
- W1658011747 cites W2066142251 @default.
- W1658011747 cites W2068358300 @default.
- W1658011747 cites W2070082950 @default.
- W1658011747 cites W2071036734 @default.
- W1658011747 cites W2072472799 @default.
- W1658011747 cites W2077250354 @default.
- W1658011747 cites W2078652488 @default.
- W1658011747 cites W2083592023 @default.
- W1658011747 cites W2085082922 @default.
- W1658011747 cites W2095166308 @default.
- W1658011747 cites W2098436412 @default.
- W1658011747 cites W2101859467 @default.
- W1658011747 cites W2102226328 @default.
- W1658011747 cites W2103002807 @default.
- W1658011747 cites W2109092337 @default.
- W1658011747 cites W2114460420 @default.
- W1658011747 cites W2121634203 @default.
- W1658011747 cites W2124797574 @default.
- W1658011747 cites W2126404692 @default.
- W1658011747 cites W2127432085 @default.
- W1658011747 cites W2133855261 @default.
- W1658011747 cites W2134139040 @default.
- W1658011747 cites W2139431409 @default.
- W1658011747 cites W2139463994 @default.
- W1658011747 cites W2144081223 @default.
- W1658011747 cites W2146157811 @default.
- W1658011747 cites W2150454530 @default.
- W1658011747 cites W2155610392 @default.
- W1658011747 cites W2156571424 @default.
- W1658011747 cites W2157709441 @default.
- W1658011747 cites W4238658571 @default.
- W1658011747 doi "https://doi.org/10.1016/j.str.2015.08.008" @default.
- W1658011747 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4788500" @default.
- W1658011747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26365802" @default.
- W1658011747 hasPublicationYear "2015" @default.
- W1658011747 type Work @default.
- W1658011747 sameAs 1658011747 @default.
- W1658011747 citedByCount "52" @default.
- W1658011747 countsByYear W16580117472015 @default.
- W1658011747 countsByYear W16580117472016 @default.
- W1658011747 countsByYear W16580117472017 @default.
- W1658011747 countsByYear W16580117472018 @default.
- W1658011747 countsByYear W16580117472019 @default.
- W1658011747 countsByYear W16580117472020 @default.
- W1658011747 countsByYear W16580117472021 @default.
- W1658011747 countsByYear W16580117472022 @default.
- W1658011747 countsByYear W16580117472023 @default.
- W1658011747 crossrefType "journal-article" @default.
- W1658011747 hasAuthorship W1658011747A5002229441 @default.
- W1658011747 hasAuthorship W1658011747A5008383835 @default.
- W1658011747 hasAuthorship W1658011747A5009326851 @default.
- W1658011747 hasAuthorship W1658011747A5020500579 @default.