Matches in SemOpenAlex for { <https://semopenalex.org/work/W1658798081> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1658798081 endingPage "6" @default.
- W1658798081 startingPage "1" @default.
- W1658798081 abstract "[Objective] The study was to supply the basis on developing a new type of MD vaccine. [Method] HSP108 gene was amplified by RT-PCR from SPF chicken liver,and MDV gB CTL epitope amplified by PCR.The fusion gene for expressing gB CTL epitope and chicken HSP108 was obtained by fusing the gene fragment encoding a MDV gB CTL epitope to 5'end of chicken HSP108 gene.And the fusion gene was inserted into pET-28a to construct the recombinant plasmid,named pET-gBHSP108.The recombinant protein named rgBHSP108 was analyzed by SDS-PAGE,solubilized in urea (8 mol/L),purified by His·Bind affinity chromatography and identified by western blotting. [Result] The chicken HSP108,a fragment of about 2.4 kb in length and MDV gB CTL epitope,a fragment of about 330bp were successfully obtained.The cloned HSP108 gene showed 99.7% homology with the HSP108 gene of chicken oviduct (GenBank Accession No.NM 178423),and 99.0% homology with the HSP108 gene of chicken liver (GenBank Accession No. AF 387865) at nucleotide levels.The fusion gene rgBHSP108 was successfully constructed,and rgBHSP108 was expressed as inclusion body and successfully purified. Immunoblotting analysis showed that the expressed fusion protein was recognized by antibody against MDV gB CTL epitope. [Conclusion] This study successfully constructed a fusion gene encoding chicken HSP108 gene and gB CTL epitope gene of MDV.And the fusion gene was successfully expressed by E.coli." @default.
- W1658798081 created "2016-06-24" @default.
- W1658798081 creator A5003941115 @default.
- W1658798081 creator A5019314361 @default.
- W1658798081 creator A5024654420 @default.
- W1658798081 creator A5054802652 @default.
- W1658798081 creator A5083972651 @default.
- W1658798081 creator A5089551012 @default.
- W1658798081 date "2009-01-01" @default.
- W1658798081 modified "2023-09-24" @default.
- W1658798081 title "Construction and prokaryotic expression of the fusion protein gene encoding MDV gB CTL epitope and chicken HSP108." @default.
- W1658798081 hasPublicationYear "2009" @default.
- W1658798081 type Work @default.
- W1658798081 sameAs 1658798081 @default.
- W1658798081 citedByCount "0" @default.
- W1658798081 crossrefType "journal-article" @default.
- W1658798081 hasAuthorship W1658798081A5003941115 @default.
- W1658798081 hasAuthorship W1658798081A5019314361 @default.
- W1658798081 hasAuthorship W1658798081A5024654420 @default.
- W1658798081 hasAuthorship W1658798081A5054802652 @default.
- W1658798081 hasAuthorship W1658798081A5083972651 @default.
- W1658798081 hasAuthorship W1658798081A5089551012 @default.
- W1658798081 hasConcept C104317684 @default.
- W1658798081 hasConcept C111829193 @default.
- W1658798081 hasConcept C123894998 @default.
- W1658798081 hasConcept C147483822 @default.
- W1658798081 hasConcept C147969180 @default.
- W1658798081 hasConcept C150194340 @default.
- W1658798081 hasConcept C153911025 @default.
- W1658798081 hasConcept C159654299 @default.
- W1658798081 hasConcept C165525559 @default.
- W1658798081 hasConcept C167672396 @default.
- W1658798081 hasConcept C195616568 @default.
- W1658798081 hasConcept C26260077 @default.
- W1658798081 hasConcept C40767141 @default.
- W1658798081 hasConcept C54355233 @default.
- W1658798081 hasConcept C79029880 @default.
- W1658798081 hasConcept C86803240 @default.
- W1658798081 hasConceptScore W1658798081C104317684 @default.
- W1658798081 hasConceptScore W1658798081C111829193 @default.
- W1658798081 hasConceptScore W1658798081C123894998 @default.
- W1658798081 hasConceptScore W1658798081C147483822 @default.
- W1658798081 hasConceptScore W1658798081C147969180 @default.
- W1658798081 hasConceptScore W1658798081C150194340 @default.
- W1658798081 hasConceptScore W1658798081C153911025 @default.
- W1658798081 hasConceptScore W1658798081C159654299 @default.
- W1658798081 hasConceptScore W1658798081C165525559 @default.
- W1658798081 hasConceptScore W1658798081C167672396 @default.
- W1658798081 hasConceptScore W1658798081C195616568 @default.
- W1658798081 hasConceptScore W1658798081C26260077 @default.
- W1658798081 hasConceptScore W1658798081C40767141 @default.
- W1658798081 hasConceptScore W1658798081C54355233 @default.
- W1658798081 hasConceptScore W1658798081C79029880 @default.
- W1658798081 hasConceptScore W1658798081C86803240 @default.
- W1658798081 hasIssue "5" @default.
- W1658798081 hasLocation W16587980811 @default.
- W1658798081 hasOpenAccess W1658798081 @default.
- W1658798081 hasPrimaryLocation W16587980811 @default.
- W1658798081 hasRelatedWork W2178538623 @default.
- W1658798081 hasRelatedWork W2349741016 @default.
- W1658798081 hasRelatedWork W2352490840 @default.
- W1658798081 hasRelatedWork W2352954913 @default.
- W1658798081 hasRelatedWork W2362141850 @default.
- W1658798081 hasRelatedWork W2364359800 @default.
- W1658798081 hasRelatedWork W2370510424 @default.
- W1658798081 hasRelatedWork W2372925135 @default.
- W1658798081 hasRelatedWork W2375061447 @default.
- W1658798081 hasRelatedWork W2375218553 @default.
- W1658798081 hasRelatedWork W2376316271 @default.
- W1658798081 hasRelatedWork W2381069110 @default.
- W1658798081 hasRelatedWork W2381310994 @default.
- W1658798081 hasRelatedWork W2383265393 @default.
- W1658798081 hasRelatedWork W2386670686 @default.
- W1658798081 hasRelatedWork W2386842231 @default.
- W1658798081 hasRelatedWork W2389092343 @default.
- W1658798081 hasRelatedWork W2392317406 @default.
- W1658798081 hasRelatedWork W3146642016 @default.
- W1658798081 hasRelatedWork W2398192313 @default.
- W1658798081 hasVolume "37" @default.
- W1658798081 isParatext "false" @default.
- W1658798081 isRetracted "false" @default.
- W1658798081 magId "1658798081" @default.
- W1658798081 workType "article" @default.