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• W165943415 abstract "The current investigation reports the evaluation and identification of genetic diversity in P. ciliata using the RAPD assay. Eighteen random decamer primers were used to assess variation within twenty five different clones, representing various provenances from the Himalayan region. A total of 159 amplification products were obtained of which 111 were polymorphic while the remaining were monomorphic in nature. Informative primers producing high multiplex ratio were identified from the study. The potential utility of these primers for large scale screening of germplasm and designing conservation strategies in the species has been discussed. The JACCARD'S similarity coefficient and the UPGMA clustering method were employed to construct the phylogenetic tree. The dendrogram revealed a high level of variation between the clones which was found to lie in accordance to the diversity observed using morphological data. Two distinct clusters namely C1 and C2 were identified. The cluster C1 comprised of twenty three of the twenty five accessions and was thus designated to be the major cluster while C2 consisted of only two clones and was thus considered to be a minor cluster. The major cluster C1 was comprised of distinct sub-clusters which were found to be in concordance to their geographical distribution. Highest similarity within the major cluster was detected between the clones Katrain and Karain Bihal while the clone Lidder was found to be the most distinct. Bootstrap analysis and principal coordinate (PCO) analysis was performed which supported the pattern of clustering in the dendrogram. The clustering of the other clones in relation to their geographical location has been discussed." @default.
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• W165943415 date "2000-01-01" @default.
• W165943415 modified "2023-09-22" @default.
• W165943415 title "Evaluation of genetic diversity in the Himalayan poplar using RAPD markers." @default.
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