Matches in SemOpenAlex for { <https://semopenalex.org/work/W166121322> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W166121322 endingPage "412" @default.
- W166121322 startingPage "405" @default.
- W166121322 abstract "When a negatively twisted covalently closed DNA is annealed with single-stranded fragments of the same DNA, under proper conditions a loop (or loops) may form by the disruption of a segment (or segments) of base pairs between the complementary strands of the covalently closed DNA, and the formation of base pairs between the strands of the covalently closed DNA and the single-stranded fragments. Since such a process involves essentially no net gain or loss of the number of base pairs, it is driven by the free energy favoring the reduction of the number of superhelical turns. If the fragments are sufficiently long or are present at a sufficiently high concentration during annealing, the most stable product between a covalently closed DNA and the DNA fragments (under conditions favoring the formation of double-stranded DNA) is a looped molecule devoid of superhelical turns. The size of the looped region or regions, which can be measured by electron microscopy, provides a way to determine the degree of superhelicity of the covalently closed DNA in the absence of the fragments. When this is compared with the degree of superhelicity of the covalently closed DNA determined by titration with the intercalative dye ethidium, the unwinding angle of the DNA double helix due to the intercalation of an ethidium can be calculated. Such measurements were done on two samples of phage PM2 DNA with different extents of supercoiling. The results are in agreement with the value 26° obtained recently by alkaline titration of covalently closed PM2 DNA samples in CsCl density gradients (Wang, J.C., (1974) J. Mol. Biol. 89, 783–801)." @default.
- W166121322 created "2016-06-24" @default.
- W166121322 creator A5011866089 @default.
- W166121322 creator A5057145998 @default.
- W166121322 date "1975-07-01" @default.
- W166121322 modified "2023-10-16" @default.
- W166121322 title "On the degree of unwinding of the DNA helix by ethidium" @default.
- W166121322 cites W1487427081 @default.
- W166121322 cites W1566605849 @default.
- W166121322 cites W1579827550 @default.
- W166121322 cites W1580329651 @default.
- W166121322 cites W1968430726 @default.
- W166121322 cites W1968713355 @default.
- W166121322 cites W1972220947 @default.
- W166121322 cites W1985855529 @default.
- W166121322 cites W1991803425 @default.
- W166121322 cites W2023569517 @default.
- W166121322 cites W2032067706 @default.
- W166121322 cites W2033202837 @default.
- W166121322 cites W2047632882 @default.
- W166121322 cites W2048429828 @default.
- W166121322 cites W2052580588 @default.
- W166121322 cites W2170193782 @default.
- W166121322 cites W2406930389 @default.
- W166121322 cites W258061546 @default.
- W166121322 doi "https://doi.org/10.1016/0005-2787(75)90064-7" @default.
- W166121322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1148245" @default.
- W166121322 hasPublicationYear "1975" @default.
- W166121322 type Work @default.
- W166121322 sameAs 166121322 @default.
- W166121322 citedByCount "62" @default.
- W166121322 countsByYear W1661213222012 @default.
- W166121322 countsByYear W1661213222013 @default.
- W166121322 countsByYear W1661213222016 @default.
- W166121322 countsByYear W1661213222020 @default.
- W166121322 countsByYear W1661213222021 @default.
- W166121322 crossrefType "journal-article" @default.
- W166121322 hasAuthorship W166121322A5011866089 @default.
- W166121322 hasAuthorship W166121322A5057145998 @default.
- W166121322 hasConcept C12554922 @default.
- W166121322 hasConcept C137824038 @default.
- W166121322 hasConcept C158456008 @default.
- W166121322 hasConcept C172455284 @default.
- W166121322 hasConcept C178790620 @default.
- W166121322 hasConcept C180577832 @default.
- W166121322 hasConcept C184866935 @default.
- W166121322 hasConcept C185581394 @default.
- W166121322 hasConcept C185592680 @default.
- W166121322 hasConcept C18903297 @default.
- W166121322 hasConcept C2778530040 @default.
- W166121322 hasConcept C2779965526 @default.
- W166121322 hasConcept C552990157 @default.
- W166121322 hasConcept C55493867 @default.
- W166121322 hasConcept C73573662 @default.
- W166121322 hasConcept C8010536 @default.
- W166121322 hasConcept C86803240 @default.
- W166121322 hasConceptScore W166121322C12554922 @default.
- W166121322 hasConceptScore W166121322C137824038 @default.
- W166121322 hasConceptScore W166121322C158456008 @default.
- W166121322 hasConceptScore W166121322C172455284 @default.
- W166121322 hasConceptScore W166121322C178790620 @default.
- W166121322 hasConceptScore W166121322C180577832 @default.
- W166121322 hasConceptScore W166121322C184866935 @default.
- W166121322 hasConceptScore W166121322C185581394 @default.
- W166121322 hasConceptScore W166121322C185592680 @default.
- W166121322 hasConceptScore W166121322C18903297 @default.
- W166121322 hasConceptScore W166121322C2778530040 @default.
- W166121322 hasConceptScore W166121322C2779965526 @default.
- W166121322 hasConceptScore W166121322C552990157 @default.
- W166121322 hasConceptScore W166121322C55493867 @default.
- W166121322 hasConceptScore W166121322C73573662 @default.
- W166121322 hasConceptScore W166121322C8010536 @default.
- W166121322 hasConceptScore W166121322C86803240 @default.
- W166121322 hasIssue "4" @default.
- W166121322 hasLocation W1661213221 @default.
- W166121322 hasLocation W1661213222 @default.
- W166121322 hasOpenAccess W166121322 @default.
- W166121322 hasPrimaryLocation W1661213221 @default.
- W166121322 hasRelatedWork W1589561038 @default.
- W166121322 hasRelatedWork W1975030039 @default.
- W166121322 hasRelatedWork W1992827354 @default.
- W166121322 hasRelatedWork W2046850778 @default.
- W166121322 hasRelatedWork W2057413467 @default.
- W166121322 hasRelatedWork W2234964239 @default.
- W166121322 hasRelatedWork W2402462105 @default.
- W166121322 hasRelatedWork W2926044694 @default.
- W166121322 hasRelatedWork W2986503575 @default.
- W166121322 hasRelatedWork W3141616521 @default.
- W166121322 hasVolume "395" @default.
- W166121322 isParatext "false" @default.
- W166121322 isRetracted "false" @default.
- W166121322 magId "166121322" @default.
- W166121322 workType "article" @default.