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- W166189471 abstract "This chapter presents detailed protocols for the maintenance of human embryonic stem (hES) cells in feeder-free conditions and then briefly describes the methods for their characterization. Because of their remarkable proliferative capacity and differentiation potential, hES cells may provide a source of cells for cell therapies, drug screening, and functional genomics applications. Derivation of hES cell lines has been accomplished in several laboratories by culturing cells from the inner cell mass of preimplantation embryos on mouse or human embryonic feeder cells. Findings indicate that hES cells require both soluble factors and matrix proteins. The cells maintained in feeder-free conditions can be induced to differentiate using specific culture conditions into neural progenitors, cardiomyocytes, trophoblast, hepatocyte-like cells, oligodendrocytes, and hematopoietic progenitors. In addition, feeder-free hES cells can be genetically modified by transfection with plasmids or infection with lentiviral vectors." @default.
- W166189471 created "2016-06-24" @default.
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- W166189471 date "2004-01-01" @default.
- W166189471 modified "2023-09-25" @default.
- W166189471 title "Feeder-free Culture" @default.
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- W166189471 doi "https://doi.org/10.1016/b978-012436643-5/50061-4" @default.
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