FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W166259743> ?p ?o ?g. }

• W166259743 endingPage "203" @default.
• W166259743 startingPage "175" @default.
• W166259743 abstract "This chapter discusses the theory and background information on separation methods and covers how the bands in gel electrophoresis and the peaks in capillary electrophoresis are actually generated and detected. To distinguish the various molecules from one another, a separation step is required to pull the different-sized fragments apart. The separation is typically performed by a process known as electrophoresis and is either conducted in a slab-gel or capillary environment. Two types of gels are commonly used in molecular biology and forensic DNA laboratories today to achieve DNA separations. Agarose gels have fairly large pore sizes and are used for separating larger DNA molecules, while polyacrylamide gels are used to obtain high-resolution separations for smaller DNA molecules, usually below 500 or 1000 bp. Under normal conditions, the two complementary strands of DNA remain together, viz., native and denaturing. The chapter explains capillary electrophoresis's advantages, disadvantages, and components. Two primary mechanisms for DNA separations through gel pores have been described: the Ogston model and reptation. The chapter describes methods and means for detecting DNA. The focus is on fluorescence detection as it is now the dominant method utilized by the forensic DNA community. The most popular detection platforms today for short tandem repeat (STR) analysis are the ABI Prism 310 Genetic Analyzer and the ABI 3100 Genetic Analyzer multicapillary system. The end result of the STR allele separation and detection steps described in the chapter is an electronic data file containing an electropherogram with many peaks. This electropherogram must be interpreted to determine the STR alleles present in the DNA sample being analyzed." @default.
• W166259743 created "2016-06-24" @default.
• W166259743 creator A5068267685 @default.
• W166259743 date "2010-01-01" @default.
• W166259743 modified "2023-09-26" @default.
• W166259743 title "Fundamentals of DNA Separation and Detection" @default.
• W166259743 cites W113392466 @default.
• W166259743 cites W1489039218 @default.
• W166259743 cites W1500909078 @default.
• W166259743 cites W1509391050 @default.
• W166259743 cites W173400113 @default.
• W166259743 cites W1967308663 @default.
• W166259743 cites W1974670959 @default.
• W166259743 cites W1978807770 @default.
• W166259743 cites W1980877885 @default.
• W166259743 cites W1985435819 @default.
• W166259743 cites W1988676851 @default.
• W166259743 cites W1993935432 @default.
• W166259743 cites W2000648436 @default.
• W166259743 cites W2017121399 @default.
• W166259743 cites W2023125921 @default.
• W166259743 cites W2033713657 @default.
• W166259743 cites W2034710803 @default.
• W166259743 cites W2037351794 @default.
• W166259743 cites W2041716520 @default.
• W166259743 cites W2075196800 @default.
• W166259743 cites W2079623673 @default.
• W166259743 cites W2090120359 @default.
• W166259743 cites W2090908773 @default.
• W166259743 cites W2094525645 @default.
• W166259743 cites W2100109836 @default.
• W166259743 cites W2101351297 @default.
• W166259743 cites W2106279020 @default.
• W166259743 cites W2119333950 @default.
• W166259743 cites W2124915444 @default.
• W166259743 cites W2129370721 @default.
• W166259743 cites W2139338922 @default.
• W166259743 cites W2144450625 @default.
• W166259743 cites W2156896172 @default.
• W166259743 cites W2158815738 @default.
• W166259743 cites W2163827341 @default.
• W166259743 cites W2397985225 @default.
• W166259743 cites W271066252 @default.
• W166259743 cites W283902625 @default.
• W166259743 doi "https://doi.org/10.1016/b978-0-12-374999-4.00009-6" @default.
• W166259743 hasPublicationYear "2010" @default.
• W166259743 type Work @default.
• W166259743 sameAs 166259743 @default.
• W166259743 citedByCount "3" @default.
• W166259743 countsByYear W1662597432014 @default.
• W166259743 countsByYear W1662597432016 @default.
• W166259743 countsByYear W1662597432022 @default.
• W166259743 crossrefType "book-chapter" @default.
• W166259743 hasAuthorship W166259743A5068267685 @default.
• W166259743 hasConcept C185592680 @default.
• W166259743 hasConcept C190911170 @default.
• W166259743 hasConcept C2778271344 @default.
• W166259743 hasConcept C2778476535 @default.
• W166259743 hasConcept C2900643 @default.
• W166259743 hasConcept C40250595 @default.
• W166259743 hasConcept C43617362 @default.
• W166259743 hasConcept C44995494 @default.
• W166259743 hasConcept C54355233 @default.
• W166259743 hasConcept C552990157 @default.
• W166259743 hasConcept C86803240 @default.
• W166259743 hasConceptScore W166259743C185592680 @default.
• W166259743 hasConceptScore W166259743C190911170 @default.
• W166259743 hasConceptScore W166259743C2778271344 @default.
• W166259743 hasConceptScore W166259743C2778476535 @default.
• W166259743 hasConceptScore W166259743C2900643 @default.
• W166259743 hasConceptScore W166259743C40250595 @default.
• W166259743 hasConceptScore W166259743C43617362 @default.
• W166259743 hasConceptScore W166259743C44995494 @default.
• W166259743 hasConceptScore W166259743C54355233 @default.
• W166259743 hasConceptScore W166259743C552990157 @default.
• W166259743 hasConceptScore W166259743C86803240 @default.
• W166259743 hasLocation W1662597431 @default.
• W166259743 hasOpenAccess W166259743 @default.
• W166259743 hasPrimaryLocation W1662597431 @default.
• W166259743 hasRelatedWork W1895397495 @default.
• W166259743 hasRelatedWork W2022779264 @default.
• W166259743 hasRelatedWork W2052930673 @default.
• W166259743 hasRelatedWork W2071577844 @default.
• W166259743 hasRelatedWork W2082287188 @default.
• W166259743 hasRelatedWork W2085130499 @default.
• W166259743 hasRelatedWork W2097129286 @default.
• W166259743 hasRelatedWork W23577426 @default.
• W166259743 hasRelatedWork W2411247343 @default.
• W166259743 hasRelatedWork W33007423 @default.
• W166259743 isParatext "false" @default.
• W166259743 isRetracted "false" @default.
• W166259743 magId "166259743" @default.
• W166259743 workType "book-chapter" @default.