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- W166263162 abstract "Genomic instability has been widely observed in in vitro-derived plants; it is proposed to result from the break-down of normal cellular control (Phillips et al, 1994). Following DNA introduction into scutella directly or embryogenic callus derived from the scutella of immature embryos (IEs), numerous transgenic barley lines were produced (Wan and Lemaux, 1994; Lemaux et al, 1996). Evaluation of these lines revealed severe reduction in agronomic performance in field tests (Bregitzer et al., 1998) and instability of the transgene and its expression (Bregitzer et al., unpublished data). We propose that poor performance may be in part due to in vitro-induced genetic or epigenetic variability. Plants derived from alternative culture methods were analyzed to study the effects of these methods on genomic stability. Plants were regenerated from tissue obtained using methods of standard embryogenic culture (Wan and Lemaux 1994), modified embryogenic callus (Cho et al., 1998) and cultured shoot meristems. Initial results with plants derived from the various methods will be compared relative to methylation polymorphism and field performance." @default.
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- W166263162 date "1999-01-01" @default.
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- W166263162 title "Comparative analysis of genomic DNA methylation status and field performance of plants derived from embryogenic calli and shoot meristematic cultures" @default.
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- W166263162 doi "https://doi.org/10.1007/978-94-011-4661-6_62" @default.
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