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• W1662956456 abstract "용존산소(DO)의 급격한 증가에 순응된 꺽지(Coreoperca herzi)와 모래무지(Pseudogobio esocinus) 조직내 젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH)의 대사와 <TEX>$C_4$</TEX> 동위효소를 확인하였다. DO 18 ppm에 갑작스럽게 순응시 꺽지 LDH활성은 뇌와 간조직에서 각각 <TEX>$35-39%$</TEX> 변화되었고, 다른 조직에서는 <TEX>$20%$</TEX>이내로 변화가 적었다. 골격근조직은 LDH <TEX>$A_4$</TEX> 동위효소가 증가되고 하부단위체 C를 포함하는 동위효소는 감소되었다. 심장조직은 <TEX>$B_4$</TEX> 동위효소가 조금 증가되었다. 신장조직도 <TEX>$B_4$</TEX>가 증가하고 눈 조직은 eye-specific <TEX>$C_4$</TEX>와 C hybrid가 감소되어 조절되었다. DO 증가에 갑작스럽게 순응한 모래무지 간조직에서 LDH 활성은 30분에서 급격하게 <TEX>$150%$</TEX>이상 크게 증가되었고, 다른 조직에서는 <TEX>$70%$</TEX> 이상 변화되었다. 그리고 골격근조직은 <TEX>$A_4$</TEX> 동위효소가 증가하고 다른 조직들에서는 <TEX>$B_4$</TEX>가 증가되었으며 특히 간 조직의 대사는 liver-specific <TEX>$C_4$</TEX>가 증가하고 <TEX>$A_4$</TEX> 는 감소되어 조절되었다. 그러나 꺽지 눈 조직의 대사는 LDH 활성이 감소하고 eye-specific <TEX>$C_4$</TEX> 동위효소가 감소되어 조절되었다. 따라서 DO증가에 순응시 모래무지는 꺽지에 비해 LDH 활성이 크게 증가되었고 동위효소의 변화정도도 크게 나타났으며, eye-spe-cific <TEX>$C_4$</TEX>와 liver-specific <TEX>$C_4$</TEX> 동위효소는 lactate oxidase로서 대사를 조절하였다. 그러므로 환경변화에 순응하여 나타나는 대사는 종이 이전에 어떤 서식조건에 적응되었는가에 따라 다른 것으로 사료된다. The metabolism of lactate dehydrogenase (EC 1.1.1.27, LDH) and <TEX>$C_4$</TEX> isozyme were studied in tissues of Coreoperca herzi and Pseudogobio esocinus acclimated to rapid increase of dissolved oxygen (DO). In C. herzi the LDH activity was changed <TEX>$35-39%$</TEX> in brain and liver tissues, and within <TEX>$20%$</TEX> in other tissues. The <TEX>$B_4$</TEX> isozyme was increased and isozyme containing subunit C was decreased in muscle tissue. The <TEX>$B_4$</TEX> isozyme was increased in heart and kidney. In P. esocinus, the LDH activity in liver tissues was largely increased to <TEX>$150%$</TEX> for 30 minute and <TEX>$70%$</TEX> in other tissues. The <TEX>$A_4$</TEX> isozyme was increased in muscle and <TEX>$B_4$</TEX> isozyme was increased in other tissues. Especially, the metabolism of liver tissue in P. esocinus was regulated by increasing liver-specific <TEX>$C_4$</TEX> and decreasing <TEX>$A_4$</TEX> isozyme. But the metabolism of eye tissue in C. herzi was regulated by decreasing LDH activity and eye-specific <TEX>$C_4$</TEX> isozyme. The LDH activity and LDH isozyme in P. esocinus were largely increased than C. herzi acclimated to rapid increase of DO. And eye-specific <TEX>$C_4$</TEX> and liver-specific <TEX>$C_4$</TEX> isozymes played role as lactate oxidase. Therefore, the response of species acclimated to rapid increase of DO seems to be variable, perhaps due to prior exposure to environmental conditions." @default.
• W1662956456 created "2016-06-24" @default.
• W1662956456 date "2005-02-01" @default.
• W1662956456 modified "2023-09-26" @default.
• W1662956456 title "Changes of Activities and Isozymes of Lactate Dehydrogenase in Coreoperca herzi and Pseudogobio esocinus Acclimated to Rapid Increase of Dissolved Oxygen" @default.
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• W1662956456 doi "https://doi.org/10.5352/jls.2005.15.1.071" @default.
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