FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1663562973> ?p ?o ?g. }

• W1663562973 endingPage "2815" @default.
• W1663562973 startingPage "2807" @default.
• W1663562973 abstract "Brome mosaic virus (BMV) genomic RNA2 encodes the 94-kDa 2a protein, which is one of two BMV nonstructural proteins required for RNA replication and subgenomic mRNA transcription. 2a contains a central polymeraselike region, which has extensive sequence similarity with the Sindbis virus nsP4 and tobacco mosaic virus (TMV) 183-kDa replication proteins, and also contains N- and C-terminal flanking segments without counterparts in the Sindbis virus and TMV nonstructural proteins. To further investigate the roles of the central and flanking segments in 2a, we have constructed a series of deletion and frameshift mutants in a biologically active BMV RNA2 cDNA clone and tested their ability to support viral RNA replication in barley protoplasts and systemic infection in whole barley plants. The entire 125-amino-acid C-terminal segment following the polymeraselike region was dispensable for RNA replication and transcription. Within the 200-amino-acid N-terminal flanking segment, deletion of the first 50 residues dramatically reduced genomic and subgenomic RNA accumulation, and deletion of 100 or more residues abolished detectable RNA synthesis. All mutations removing residues from the central polymeraselike domain also blocked RNA replication in trans. Sequences required in cis for RNA2 replication or stability were found to occur within the first 300 nucleotides of the 2a coding region. In whole barley plants, systemic infection was inhibited even by 2a deletions that supported strong RNA replication in protoplasts. Some replication-competent 2a variants failed to spread to uninoculated leaves, while other showed 10- to 500-fold-reduced virus yield in both inoculated and uninoculated leaves. These reductions were not due to any defects in RNA2 encapsidation." @default.
• W1663562973 created "2016-06-24" @default.
• W1663562973 creator A5009117570 @default.
• W1663562973 creator A5026899432 @default.
• W1663562973 creator A5088338902 @default.
• W1663562973 date "1991-06-01" @default.
• W1663562973 modified "2023-09-26" @default.
• W1663562973 title "Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread" @default.
• W1663562973 cites W115032764 @default.
• W1663562973 cites W1532353118 @default.
• W1663562973 cites W1582561043 @default.
• W1663562973 cites W1589329533 @default.
• W1663562973 cites W1628437610 @default.
• W1663562973 cites W1767303762 @default.
• W1663562973 cites W1859606218 @default.
• W1663562973 cites W1870498429 @default.
• W1663562973 cites W1902051377 @default.
• W1663562973 cites W1917399449 @default.
• W1663562973 cites W1964689773 @default.
• W1663562973 cites W1980821646 @default.
• W1663562973 cites W1987408163 @default.
• W1663562973 cites W1989933096 @default.
• W1663562973 cites W2005402262 @default.
• W1663562973 cites W2021603377 @default.
• W1663562973 cites W2022586495 @default.
• W1663562973 cites W2022765393 @default.
• W1663562973 cites W2024460002 @default.
• W1663562973 cites W2026260520 @default.
• W1663562973 cites W2027271983 @default.
• W1663562973 cites W2028098813 @default.
• W1663562973 cites W2029449095 @default.
• W1663562973 cites W2037720398 @default.
• W1663562973 cites W2044524906 @default.
• W1663562973 cites W2045666611 @default.
• W1663562973 cites W2057345278 @default.
• W1663562973 cites W2069236976 @default.
• W1663562973 cites W2075704510 @default.
• W1663562973 cites W2083188919 @default.
• W1663562973 cites W2084162268 @default.
• W1663562973 cites W2085825896 @default.
• W1663562973 cites W2090477243 @default.
• W1663562973 cites W2091265011 @default.
• W1663562973 cites W2095433332 @default.
• W1663562973 cites W2099028920 @default.
• W1663562973 cites W2101152547 @default.
• W1663562973 cites W2113806981 @default.
• W1663562973 cites W2117708081 @default.
• W1663562973 cites W2118120797 @default.
• W1663562973 cites W2123792518 @default.
• W1663562973 cites W2129468535 @default.
• W1663562973 cites W2132783825 @default.
• W1663562973 cites W2161888878 @default.
• W1663562973 cites W2424380432 @default.
• W1663562973 cites W2888511473 @default.
• W1663562973 cites W926400198 @default.
• W1663562973 doi "https://doi.org/10.1128/jvi.65.6.2807-2815.1991" @default.
• W1663562973 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/240898" @default.
• W1663562973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2033655" @default.
• W1663562973 hasPublicationYear "1991" @default.
• W1663562973 type Work @default.
• W1663562973 sameAs 1663562973 @default.
• W1663562973 citedByCount "117" @default.
• W1663562973 countsByYear W16635629732012 @default.
• W1663562973 countsByYear W16635629732013 @default.
• W1663562973 countsByYear W16635629732016 @default.
• W1663562973 countsByYear W16635629732017 @default.
• W1663562973 countsByYear W16635629732018 @default.
• W1663562973 countsByYear W16635629732021 @default.
• W1663562973 countsByYear W16635629732023 @default.
• W1663562973 crossrefType "journal-article" @default.
• W1663562973 hasAuthorship W1663562973A5009117570 @default.
• W1663562973 hasAuthorship W1663562973A5026899432 @default.
• W1663562973 hasAuthorship W1663562973A5088338902 @default.
• W1663562973 hasBestOaLocation W16635629731 @default.
• W1663562973 hasConcept C104317684 @default.
• W1663562973 hasConcept C138885662 @default.
• W1663562973 hasConcept C140704245 @default.
• W1663562973 hasConcept C153911025 @default.
• W1663562973 hasConcept C159047783 @default.
• W1663562973 hasConcept C179926584 @default.
• W1663562973 hasConcept C189819185 @default.
• W1663562973 hasConcept C2522874641 @default.
• W1663562973 hasConcept C2777343345 @default.
• W1663562973 hasConcept C2779535198 @default.
• W1663562973 hasConcept C41258723 @default.
• W1663562973 hasConcept C41895202 @default.
• W1663562973 hasConcept C54355233 @default.
• W1663562973 hasConcept C67705224 @default.
• W1663562973 hasConcept C86803240 @default.
• W1663562973 hasConceptScore W1663562973C104317684 @default.
• W1663562973 hasConceptScore W1663562973C138885662 @default.
• W1663562973 hasConceptScore W1663562973C140704245 @default.
• W1663562973 hasConceptScore W1663562973C153911025 @default.
• W1663562973 hasConceptScore W1663562973C159047783 @default.
• W1663562973 hasConceptScore W1663562973C179926584 @default.
• W1663562973 hasConceptScore W1663562973C189819185 @default.
• W1663562973 hasConceptScore W1663562973C2522874641 @default.
• W1663562973 hasConceptScore W1663562973C2777343345 @default.

• next