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• W16636296 abstract "We describe a suspension array hybridization assay for rapid detection and identification of Salmonella and other bacterial pathogens using Luminex xMAP technology. The Luminex xMAP system allows simultaneous detection of up to 100 different targets in a single multiplexed reaction. Included in the method are the procedures for (1) design of species-specific oligonucleotide capture probes and PCR amplification primers, (2) coupling oligonucleotide capture probes to carboxylated microspheres, (3) hybridization of coupled microspheres to oligonucleotide targets, (4) production of targets from DNA samples by PCR amplification, and (5) detection of PCR-amplified targets by direct hybridization to probe-coupled microspheres. The Luminex xMAP suspension array hybridization assay is rapid, requires few sample manipulations, and provides adequate sensitivity and specificity to detect and differentiate Salmonella and nine other test organisms through direct detection of species-specific DNA sequences." @default.
• W16636296 created "2016-06-24" @default.
• W16636296 creator A5046375067 @default.
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• W16636296 date "2007-01-01" @default.
• W16636296 modified "2023-10-14" @default.
• W16636296 title "Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex® xMAP™ Suspension Array" @default.
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• W16636296 doi "https://doi.org/10.1007/978-1-59745-512-1_1" @default.
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