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- W1665572110 abstract "Publisher Summary This chapter describes work concerning the cloning and expression of Candida rugosa lipase (CRLs), which are peculiar among fungal lipases for two reasons: (1) the large number of encoding genes and (2) a deviation from the universal genetic code in the source yeast. The importance and effects of this phenomenon for lipase structure and function, as well as for the expression of cloned genes in host systems, are discussed. The three-dimensional structure of the major lipase form (CRL1) has been solved in its open and closed conformation as well as in complexes with substrate analogs, providing clues to the structure and action of this enzyme. The chapter explores the possibility of expressing CRL genes in Candida cells using the same genetic code as C. rugosa . By this means, the production and secretion of recombinant lipases are obtained. However, the uniqueness of C. rugosa in terms of the frequency of CUG, on the one hand, and its apparent use of signals different from those of other organisms, on the other hand, still requires to optimize the expression systems to be able to produce recombinant CRL on a large scale." @default.
- W1665572110 created "2016-06-24" @default.
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- W1665572110 date "1997-01-01" @default.
- W1665572110 modified "2023-09-27" @default.
- W1665572110 title "[14] Cloning, sequencing, and expression of Candida rugosa lipases" @default.
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- W1665572110 doi "https://doi.org/10.1016/s0076-6879(97)84016-5" @default.
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