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- W166563713 abstract "With heat treatment (20 degrees C for 30 min), the glucocorticoid-receptor complex becomes ‘activated’ and undergoes an increase in affinity for DNA. A two-stage procedure was used to separate sequentially the rat liver glucocorticoid-receptor complex from proteins with high and low affinity for DNA. DNA-cellulose column chromatography of unheated cytosol resulted in the retention of DNA-binding proteins, but not the unactivated receptor complex. Heat treatment of the column eluate resulted in increased affinity of the receptor complex to DNA, and chromatography on DNA-cellulose then yielded receptor complex free from proteins with low affinity for DNA. Removal of DNA-binding proteins during the first chromatographic step was critically dependent on ionic conditions and the ratio of cytosol chromatographed to DNA-cellulose. A purification of 11000-fold (85% yield) was achieved by this procedure. The partially purified receptor complex was taken up by rat liver nuclei." @default.
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- W166563713 date "1978-04-01" @default.
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- W166563713 title "Maximizing the purification of the activated glucocorticoid receptor by DNA-cellulose chromatography" @default.
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- W166563713 doi "https://doi.org/10.1042/bj1710177" @default.
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