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• W1666397029 abstract "Supernatants from apple cutin-induced cultures of the light leaf spot pathogen Pyrenopeziza brassicae were shown to contain a single esterase with a pI of approx. 4.4 and a MW of 21 kDa. Analysis of the hydrolysis products derived from enzymatic cleavage of3H labelled cutin by TLC demonstrated that these supernatants had cutinolytic activity. Treatment with the serine esterase inhibitors ebelactones A and B resulted in 50% inhibition of theP. brassicae esterase activity at concentrations of 4 and 0.4 μ g ml−1, respectively. It is therefore concluded that following induction in vitro with apple cutin, P. brassicae produces a single extracellular esterase with cutinolytic activity. A putative cutinase gene was cloned by heterologous Southern hybridization of P. brassicae genomic DNA using the Botrytis cinerea cutinase gene as a probe. The ORF (706 bp) is predicted to encode a polypeptide of 203 amino acids with a molecular weight of 20.379 kDa and a pI of 4.38. This polypeptide shows high levels of homology with the cutinases of B. cinerea and of other fungi, and contains the conserved histidine, aspartic acid and serine catalytic triad. Expression of the putative cutinase gene was shown to correlate with induction of esterase activity in vitro. SEM analysis of Brassica napus leaves infected withP. brassicae confirmed that direct penetration of the host surface occurs with no evidence of appressorial formation. The ebelactones A (100 μ g ml−1) and B (10 μ g ml−1) had no effect on germination of P. brassicae conidia in vitro; however, a marked decrease in pathogenicity of P. brassicae was observed on B. napus plants treated with 100 μ g ml−1ebelactone A or 10 μ g ml−1ebelactone B. In these plants, failed penetration was observed and the number of acervular conidiomata was greatly reduced. These results suggest a functional role for fungal cutinolytic activity in pathogenicity of P. brassicae on B. napus." @default.
• W1666397029 created "2016-06-24" @default.
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• W1666397029 date "2000-08-01" @default.
• W1666397029 modified "2023-09-26" @default.
• W1666397029 title "Evidence for a role of cutinase in pathogenicity of Pyrenopeziza brassicae on brassicas" @default.
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• W1666397029 doi "https://doi.org/10.1006/pmpp.2000.0282" @default.
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