FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1667222495> ?p ?o ?g. }

• W1667222495 endingPage "998" @default.
• W1667222495 startingPage "988" @default.
• W1667222495 abstract "These investigations are concerned with the ionic and substrate-site requirements of ribosomal ribonucleic acid (rRNA)-adenine (N 6 -) methylase of Escherichia coli B. The methylase was essentially inactive in solutions of low ionic strength. The addition of MgCl 2 (optimal at 5 mM) or; to a lesser degree, KCl (optimal at 45 mM) stimulated the rate of methylation; the combination of MgCl 2 and KCl stimulated methylation to an extent equivalent to the sum of the stimulation of each acting alone. The extent of nonspecific binding of the methylase to rRNA decreased as the ionic strength of the solution increased. In the absence of ions, dimethylsulfoxide (DMSO), a nucleic acid denaturing agent, had little influence on the rate of methylation; however, DMSO plus KCl synergistically increased both the rate and the extent of methylation to a greater degree than the combination of Mg 2+ plus K + . NH 4 + was less effective than K + , and the divalent Mg 2+ offered little stimulation. Monovalent anions (acetate, nitrate, and chloride) were equally effective, whereas divalent SO 4 2− was decidedly inhibitory. The appropriate ionic milieu of mono- and divalent cations was required to provide the appropriate conformation of the rRNA and to facilitate specific interactions of the methylase and its recognition sites in the rRNA, while decreasing nonspecific ionic binding of the methylase to rRNA. DMSO may facilitate methylation by increasing the number of substrate sites exposed in single-stranded regions of the rRNA. Nonmethylatable rRNA species served as competitive inhibitors, whereas the polyanions deoxyribonucleic acid, transfer RNA, and polyadenylic acid were inactive. Micrococcus lysodeikticus and Bacillus subtilis rRNA, methylated by the methylase, each contained two distinct heptanucleotides containing newly synthesized 6-methyladenine moieties. The data are consistent with the view that E. coli strain B possesses two species of rRNA-adenine (N 6 -) methylases, each of which recognizes a specific adenine moiety in a unique pentapurine nucleotide sequence in a single-stranded region of rRNA." @default.
• W1667222495 created "2016-06-24" @default.
• W1667222495 creator A5004975347 @default.
• W1667222495 creator A5053690926 @default.
• W1667222495 creator A5059400351 @default.
• W1667222495 date "1973-06-01" @default.
• W1667222495 modified "2023-10-16" @default.
• W1667222495 title "Ribosomal Ribonucleic Acid-Adenine (N 6 -) Methylase of Escherichia coli Strain B: Ionic and Substrate Site Requirements" @default.
• W1667222495 cites W1487915912 @default.
• W1667222495 cites W1599127531 @default.
• W1667222495 cites W1682701058 @default.
• W1667222495 cites W1895451657 @default.
• W1667222495 cites W1965802677 @default.
• W1667222495 cites W1969145343 @default.
• W1667222495 cites W1972576765 @default.
• W1667222495 cites W1980427538 @default.
• W1667222495 cites W1985386276 @default.
• W1667222495 cites W1993488588 @default.
• W1667222495 cites W1993699070 @default.
• W1667222495 cites W1998770548 @default.
• W1667222495 cites W2001243125 @default.
• W1667222495 cites W2002640247 @default.
• W1667222495 cites W2011780891 @default.
• W1667222495 cites W2023227497 @default.
• W1667222495 cites W2027695117 @default.
• W1667222495 cites W2044809585 @default.
• W1667222495 cites W2058111023 @default.
• W1667222495 cites W2072925604 @default.
• W1667222495 cites W2081393868 @default.
• W1667222495 cites W2081861752 @default.
• W1667222495 cites W2082929049 @default.
• W1667222495 cites W2083945806 @default.
• W1667222495 cites W2089977445 @default.
• W1667222495 cites W2147366434 @default.
• W1667222495 cites W2332302461 @default.
• W1667222495 cites W2407584312 @default.
• W1667222495 cites W2943990977 @default.
• W1667222495 cites W4236799860 @default.
• W1667222495 cites W4240860700 @default.
• W1667222495 cites W72922508 @default.
• W1667222495 cites W980031318 @default.
• W1667222495 doi "https://doi.org/10.1128/jb.114.3.988-998.1973" @default.
• W1667222495 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/285355" @default.
• W1667222495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4576414" @default.
• W1667222495 hasPublicationYear "1973" @default.
• W1667222495 type Work @default.
• W1667222495 sameAs 1667222495 @default.
• W1667222495 citedByCount "3" @default.
• W1667222495 crossrefType "journal-article" @default.
• W1667222495 hasAuthorship W1667222495A5004975347 @default.
• W1667222495 hasAuthorship W1667222495A5053690926 @default.
• W1667222495 hasAuthorship W1667222495A5059400351 @default.
• W1667222495 hasBestOaLocation W16672224951 @default.
• W1667222495 hasConcept C104317684 @default.
• W1667222495 hasConcept C153911025 @default.
• W1667222495 hasConcept C178790620 @default.
• W1667222495 hasConcept C181199279 @default.
• W1667222495 hasConcept C184651966 @default.
• W1667222495 hasConcept C185592680 @default.
• W1667222495 hasConcept C187428577 @default.
• W1667222495 hasConcept C18903297 @default.
• W1667222495 hasConcept C2777289219 @default.
• W1667222495 hasConcept C33288867 @default.
• W1667222495 hasConcept C547475151 @default.
• W1667222495 hasConcept C552990157 @default.
• W1667222495 hasConcept C55493867 @default.
• W1667222495 hasConcept C67705224 @default.
• W1667222495 hasConcept C67905577 @default.
• W1667222495 hasConcept C74884574 @default.
• W1667222495 hasConcept C86803240 @default.
• W1667222495 hasConcept C91965660 @default.
• W1667222495 hasConceptScore W1667222495C104317684 @default.
• W1667222495 hasConceptScore W1667222495C153911025 @default.
• W1667222495 hasConceptScore W1667222495C178790620 @default.
• W1667222495 hasConceptScore W1667222495C181199279 @default.
• W1667222495 hasConceptScore W1667222495C184651966 @default.
• W1667222495 hasConceptScore W1667222495C185592680 @default.
• W1667222495 hasConceptScore W1667222495C187428577 @default.
• W1667222495 hasConceptScore W1667222495C18903297 @default.
• W1667222495 hasConceptScore W1667222495C2777289219 @default.
• W1667222495 hasConceptScore W1667222495C33288867 @default.
• W1667222495 hasConceptScore W1667222495C547475151 @default.
• W1667222495 hasConceptScore W1667222495C552990157 @default.
• W1667222495 hasConceptScore W1667222495C55493867 @default.
• W1667222495 hasConceptScore W1667222495C67705224 @default.
• W1667222495 hasConceptScore W1667222495C67905577 @default.
• W1667222495 hasConceptScore W1667222495C74884574 @default.
• W1667222495 hasConceptScore W1667222495C86803240 @default.
• W1667222495 hasConceptScore W1667222495C91965660 @default.
• W1667222495 hasIssue "3" @default.
• W1667222495 hasLocation W16672224951 @default.
• W1667222495 hasLocation W16672224952 @default.
• W1667222495 hasLocation W16672224953 @default.
• W1667222495 hasOpenAccess W1667222495 @default.
• W1667222495 hasPrimaryLocation W16672224951 @default.
• W1667222495 hasRelatedWork W134745220 @default.
• W1667222495 hasRelatedWork W1480113691 @default.
• W1667222495 hasRelatedWork W1605996707 @default.

• next