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- W167026354 abstract "Abstract Results from 8 laboratories are presented for the collaborative study of a proposed method for quantitative liquid chromatographic (LC) determination of lactose purity. Each laboratory was provided with 10 blind duplicate, edible-grade lactose samples plus a lactose reference standard and a fructose internal standard. Testing required the comparison of aqueous 2% lactose sample solution against a reference standard of equal concentration and quantitation by using a fructose internal standard. The LC system specified an isocratic acetonitrilewater (80 + 20) mobile phase, flow rate 2.0 mL/min, and chromatographic separation on a carbohydrate column. Compounds were detected by using refractive index. Statistical analysis for all study data indicates excellent between-laboratory precision with a coefficient of variation (CV) range of 0.53-1.37% and an overall average of 0.977%. Statistical analysis after excluding outliers showed equally good repeatability and reproducibility of less than 1 % and 1.5% CV, respectively. The method has been adopted official first action." @default.
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- W167026354 date "1984-09-01" @default.
- W167026354 modified "2023-09-26" @default.
- W167026354 title "Liquid Chromatographic Determination of Lactose Purity: Collaborative Study" @default.
- W167026354 doi "https://doi.org/10.1093/jaoac/67.5.899" @default.
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