Matches in SemOpenAlex for { <https://semopenalex.org/work/W1671027211> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W1671027211 endingPage "2033" @default.
- W1671027211 startingPage "2022" @default.
- W1671027211 abstract "The plasma membrane proteins CD1a, CD1b and CD1c are expressed by human dendritic cells, the professional antigen‐presenting cells of the immune system, and present lipid antigens to T lymphocytes. CD1e belongs to the same family of molecules, but accumulates as a membrane‐associated form in the Golgi compartments of immature dendritic cells and as a soluble cleaved form in the lysosomes of mature dendritic cells. In lysosomes, the N‐terminal propeptide of CD1e is also cleaved, but the functional consequences of this step are unknown. Here, we investigated how the pH changes encountered during transport to lysosomes affect the structure of CD1e and its ligand‐binding properties. Circular dichroism studies demonstrated that the secondary and tertiary structures of recombinant CD1e were barely altered by pH changes. Nevertheless, at acidic pH, guanidium chloride‐induced unfolding of CD1e molecules required lower concentrations of denaturing agent. The nonfunctional L194P allelic variant was found to be structurally less stable at acidic pH than the functional forms, providing an explanation for the lack of its detection in lysosomes. The number of water‐exposed hydrophobic patches that bind 8‐anilinonaphthalene‐1‐sulfonate was higher in acidic conditions, especially for the L194P variant. CD1e molecules interacted with lipid surfaces enriched in anionic lipids, such as bis(monoacylglycero)phosphate, a late endosomal/lysosomal lipid, especially at acidic pH, or when the propeptide was present. Altogether, these data indicate that, in the late endosomes/lysosomes of DCs, the acid pH promotes the binding of lipid antigens to CD1e through increased hydrophobic and ionic interactions." @default.
- W1671027211 created "2016-06-24" @default.
- W1671027211 creator A5015974411 @default.
- W1671027211 creator A5017111874 @default.
- W1671027211 creator A5031690157 @default.
- W1671027211 creator A5050219067 @default.
- W1671027211 creator A5073919874 @default.
- W1671027211 creator A5085957231 @default.
- W1671027211 creator A5088441623 @default.
- W1671027211 date "2011-05-13" @default.
- W1671027211 modified "2023-10-18" @default.
- W1671027211 title "Increased flexibility and liposome-binding capacity of CD1e at endosomal pH" @default.
- W1671027211 cites W1522913832 @default.
- W1671027211 cites W1558247035 @default.
- W1671027211 cites W1968899948 @default.
- W1671027211 cites W2010933883 @default.
- W1671027211 cites W2027841252 @default.
- W1671027211 cites W2030210503 @default.
- W1671027211 cites W2036130970 @default.
- W1671027211 cites W2039226425 @default.
- W1671027211 cites W2054877609 @default.
- W1671027211 cites W2070459187 @default.
- W1671027211 cites W2071530096 @default.
- W1671027211 cites W2073403586 @default.
- W1671027211 cites W2078100063 @default.
- W1671027211 cites W2089645471 @default.
- W1671027211 cites W2108305553 @default.
- W1671027211 cites W2118830268 @default.
- W1671027211 cites W2120832265 @default.
- W1671027211 cites W2125738893 @default.
- W1671027211 cites W2137251331 @default.
- W1671027211 cites W2140260269 @default.
- W1671027211 cites W2143749697 @default.
- W1671027211 cites W2149138461 @default.
- W1671027211 cites W2172105693 @default.
- W1671027211 doi "https://doi.org/10.1111/j.1742-4658.2011.08118.x" @default.
- W1671027211 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21481186" @default.
- W1671027211 hasPublicationYear "2011" @default.
- W1671027211 type Work @default.
- W1671027211 sameAs 1671027211 @default.
- W1671027211 citedByCount "3" @default.
- W1671027211 countsByYear W16710272112012 @default.
- W1671027211 countsByYear W16710272112014 @default.
- W1671027211 crossrefType "journal-article" @default.
- W1671027211 hasAuthorship W1671027211A5015974411 @default.
- W1671027211 hasAuthorship W1671027211A5017111874 @default.
- W1671027211 hasAuthorship W1671027211A5031690157 @default.
- W1671027211 hasAuthorship W1671027211A5050219067 @default.
- W1671027211 hasAuthorship W1671027211A5073919874 @default.
- W1671027211 hasAuthorship W1671027211A5085957231 @default.
- W1671027211 hasAuthorship W1671027211A5088441623 @default.
- W1671027211 hasConcept C102747710 @default.
- W1671027211 hasConcept C119062480 @default.
- W1671027211 hasConcept C12554922 @default.
- W1671027211 hasConcept C133571119 @default.
- W1671027211 hasConcept C158617107 @default.
- W1671027211 hasConcept C170493617 @default.
- W1671027211 hasConcept C185154212 @default.
- W1671027211 hasConcept C185592680 @default.
- W1671027211 hasConcept C55493867 @default.
- W1671027211 hasConcept C86803240 @default.
- W1671027211 hasConcept C95444343 @default.
- W1671027211 hasConceptScore W1671027211C102747710 @default.
- W1671027211 hasConceptScore W1671027211C119062480 @default.
- W1671027211 hasConceptScore W1671027211C12554922 @default.
- W1671027211 hasConceptScore W1671027211C133571119 @default.
- W1671027211 hasConceptScore W1671027211C158617107 @default.
- W1671027211 hasConceptScore W1671027211C170493617 @default.
- W1671027211 hasConceptScore W1671027211C185154212 @default.
- W1671027211 hasConceptScore W1671027211C185592680 @default.
- W1671027211 hasConceptScore W1671027211C55493867 @default.
- W1671027211 hasConceptScore W1671027211C86803240 @default.
- W1671027211 hasConceptScore W1671027211C95444343 @default.
- W1671027211 hasIssue "12" @default.
- W1671027211 hasLocation W16710272111 @default.
- W1671027211 hasLocation W16710272112 @default.
- W1671027211 hasLocation W16710272113 @default.
- W1671027211 hasOpenAccess W1671027211 @default.
- W1671027211 hasPrimaryLocation W16710272111 @default.
- W1671027211 hasRelatedWork W1559402260 @default.
- W1671027211 hasRelatedWork W1968774879 @default.
- W1671027211 hasRelatedWork W2002985209 @default.
- W1671027211 hasRelatedWork W2032850298 @default.
- W1671027211 hasRelatedWork W2128691473 @default.
- W1671027211 hasRelatedWork W21408002 @default.
- W1671027211 hasRelatedWork W2159447933 @default.
- W1671027211 hasRelatedWork W2629150672 @default.
- W1671027211 hasRelatedWork W3181742134 @default.
- W1671027211 hasRelatedWork W4322758105 @default.
- W1671027211 hasVolume "278" @default.
- W1671027211 isParatext "false" @default.
- W1671027211 isRetracted "false" @default.
- W1671027211 magId "1671027211" @default.
- W1671027211 workType "article" @default.