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- W1673063762 abstract "The more recent development of the polymerase chain reaction (PCR) enables the amplification of target DNA in the specimen, increasing the sensitivity of the tests undertaken for diagnosis of infections by several orders of magnitude. Positive hybridization data, and in the case of PCR, demonstration in gels of the amplified target sequence, may suffice to indicate the presence of the infectious agent. The first DNA probes applied to diagnosis of mycoplasma infections consisted of cloned ribosomal RNA genes, such as the recombinant plasmid pMC5. Another class of DNA probes consisting of chromosomal segments specific for a certain mollicute species is discussed. These segments are derived from a genomic library of the specific mycoplasm. PCR tests are several orders of magnitude more sensitive than those based on direct hybridization with a DNA probe. The first major issue in the development of a PCR-based test concerns the selection of the appropriate target sequences for amplification. The sequence to be amplified can be chosen from a published mycoplasmal gene sequence or from a randomly cloned DNA fragment demonstrated to be specific for the mycoplasma to be detected. The methodology of target sequence amplification and optimization of the PCR test conditions as well as the identification of the PCR products are presented." @default.
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- W1673063762 date "1996-01-01" @default.
- W1673063762 modified "2023-09-27" @default.
- W1673063762 title "Introductory remarks" @default.
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- W1673063762 doi "https://doi.org/10.1016/b978-012583806-1/50113-9" @default.
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