FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1673269086> ?p ?o ?g. }

• W1673269086 abstract "Femtosecond stimulated Raman microscopy (FSRM) is an upcoming technique in vibrational microscopy that combines optical imaging with the chemical sensitivity of Raman scattering. It allows for the quantitative, space resolved representation of microscopic samples. In FSRM, chemical contrast relies on femtosecond stimulated Raman scattering (FSRS), a nonlinear Raman process providing signal levels, that are strongly amplified with respect to spontaneous Raman scattering. Therefore, FSRM benefits from shortened exposure times and improved signal/noise-ratios. Even though FSRS is an optically nonlinear effect, its spectral signature resembles the (polarised) spontaneous Raman spectra. Autofluorescence of the sample does not contribute to the FSRS signature. FSRM turns out to be suitable for chemical imaging of biological systems, as cells and tissues. Since their signal strengths are quite low, data with high signal/noise-ratios is essential for the specificanalysis of the sample. These requirements can be provided by FSRM. Moreover, the linear dependency of the FSRS signal on the sample concentration facilitates the quantitativeanalysis of its molecular composition. Given that tissue shows a wide heterogeneity on the molecular level, spectrally broad information, which is indeed provided by FSRM, is indispensable for chemically sensitive imaging. The intention of the present work was to establish FSRM as a tool for biological and medical imaging. FSRM is a scanning technique that relies on the nonlinear interaction of two ultrashort laser pulses with a Raman active medium. One of them is a spectrally narrow and intense picosecond pulse (Raman pump pulse), the other a spectrally broad and weak femtosecond pulse (Raman probe pulse). Both pulses are coupled collinearly into a scanning microscope where stimulated Raman scattering occurs in the sample positioned at the focus. This interaction leads to a spectral modification of the Raman probe pulse, which is recorded by the aid of a multi-channel detector. Its FSRS spectrum can be obtained by referencing the femtosecond pulse in presence of the Raman pump pulse to the one in absence. By raster-scanning the sample space-resolved FSRS spectra are retrieved. In the first FSRM setup a laser/amplifier system served as laser source. Its peak intensities are not compatible with the requirements of biological systems. Therefore a new light source has been developed for FSRM. It is based on a laser oscillator running at high repetition rate, which provides ultrashort femtosecond pulses with a spectral width over more than 3000 cm-1. These serve directly as Raman probe pulses. The Raman pump pulse is generated out of the laser spectrum by means of a ytterbium based fiber amplifier.Stimulated Raman microscopy is considered as a disturbance-free, nonlinear microscopy technique. In the context of this work, a further nonlinear contributionto the FSRS signal is reported for the first time. It shows up as a strong oscillation of the baseline in the spectrum and deteriorates the signal/noise-ratio as well as the spectral selectivity. This background can be identified as a spectral interference between the Raman probe pulse and an additional electric field, which is generated by a four-wave-mixing process between the Raman pump and Raman probe pulse. Properties and methods to suppress the spectral interferences are presented." @default.
• W1673269086 created "2016-06-24" @default.
• W1673269086 creator A5085161245 @default.
• W1673269086 date "2011-12-19" @default.
• W1673269086 modified "2023-09-27" @default.
• W1673269086 title "Spektrale Modulationen in der Femtosekunden-Stimulierten Raman-Mikroskopie" @default.
• W1673269086 cites W1242424520 @default.
• W1673269086 cites W1519847288 @default.
• W1673269086 cites W1533177992 @default.
• W1673269086 cites W1555036541 @default.
• W1673269086 cites W1576671941 @default.
• W1673269086 cites W1577199596 @default.
• W1673269086 cites W1589790730 @default.
• W1673269086 cites W1596164007 @default.
• W1673269086 cites W1617093779 @default.
• W1673269086 cites W1891609328 @default.
• W1673269086 cites W1963816408 @default.
• W1673269086 cites W1967620438 @default.
• W1673269086 cites W1968129320 @default.
• W1673269086 cites W1969235825 @default.
• W1673269086 cites W1971104329 @default.
• W1673269086 cites W1971342871 @default.
• W1673269086 cites W1972018555 @default.
• W1673269086 cites W1973107332 @default.
• W1673269086 cites W1975461562 @default.
• W1673269086 cites W1979118790 @default.
• W1673269086 cites W1981735509 @default.
• W1673269086 cites W1982606807 @default.
• W1673269086 cites W1985310175 @default.
• W1673269086 cites W1986919663 @default.
• W1673269086 cites W1987046316 @default.
• W1673269086 cites W1988306577 @default.
• W1673269086 cites W1989839235 @default.
• W1673269086 cites W1996580214 @default.
• W1673269086 cites W1997338987 @default.
• W1673269086 cites W2004128332 @default.
• W1673269086 cites W2006043357 @default.
• W1673269086 cites W2008686988 @default.
• W1673269086 cites W2010590446 @default.
• W1673269086 cites W2012064484 @default.
• W1673269086 cites W2013963499 @default.
• W1673269086 cites W2015882515 @default.
• W1673269086 cites W2016752876 @default.
• W1673269086 cites W2016941483 @default.
• W1673269086 cites W2017795302 @default.
• W1673269086 cites W2018480220 @default.
• W1673269086 cites W2020295760 @default.
• W1673269086 cites W2021964673 @default.
• W1673269086 cites W2021995235 @default.
• W1673269086 cites W2031646584 @default.
• W1673269086 cites W2032301170 @default.
• W1673269086 cites W2032629874 @default.
• W1673269086 cites W2033960109 @default.
• W1673269086 cites W2034836172 @default.
• W1673269086 cites W2036429026 @default.
• W1673269086 cites W2038703722 @default.
• W1673269086 cites W2040112768 @default.
• W1673269086 cites W2040960402 @default.
• W1673269086 cites W2044203059 @default.
• W1673269086 cites W2045407210 @default.
• W1673269086 cites W2045901799 @default.
• W1673269086 cites W2047718540 @default.
• W1673269086 cites W2049167675 @default.
• W1673269086 cites W2050103929 @default.
• W1673269086 cites W2053697885 @default.
• W1673269086 cites W2055489635 @default.
• W1673269086 cites W2055625108 @default.
• W1673269086 cites W2056223295 @default.
• W1673269086 cites W2058687177 @default.
• W1673269086 cites W2059387772 @default.
• W1673269086 cites W2060654966 @default.
• W1673269086 cites W2061524417 @default.
• W1673269086 cites W2064173118 @default.
• W1673269086 cites W2064539068 @default.
• W1673269086 cites W2069969616 @default.
• W1673269086 cites W2071274854 @default.
• W1673269086 cites W2072443063 @default.
• W1673269086 cites W2073513617 @default.
• W1673269086 cites W2075485544 @default.
• W1673269086 cites W2076425962 @default.
• W1673269086 cites W2077260443 @default.
• W1673269086 cites W2078387443 @default.
• W1673269086 cites W2078647925 @default.
• W1673269086 cites W2079201961 @default.
• W1673269086 cites W2079609666 @default.
• W1673269086 cites W2082932859 @default.
• W1673269086 cites W2083157061 @default.
• W1673269086 cites W2084490202 @default.
• W1673269086 cites W2086089711 @default.
• W1673269086 cites W2087022333 @default.
• W1673269086 cites W2089539304 @default.
• W1673269086 cites W2092007119 @default.
• W1673269086 cites W2092567220 @default.
• W1673269086 cites W2092982737 @default.
• W1673269086 cites W2096335633 @default.
• W1673269086 cites W2097578667 @default.
• W1673269086 cites W2099838183 @default.
• W1673269086 cites W2102531128 @default.
• W1673269086 cites W2102874009 @default.
• W1673269086 cites W2104159790 @default.

• next