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- W1673295677 abstract "This chapter reviews nuclease protection/solution hybridization assay, which can be used to quantitate the amount of the primary transcript, processing intermediates, or mature mRNA in the nucleus. It also discusses an intervening sequence in situ hybridization histochemistry, which can detect the level of the primary transcript in individual cell nuclei. The primary transcripts, processing intermediates, and mature mRNA from the pelleted nuclei can be purified so that it can be determined whether the experimental treatment affects RNA processing. With the availability of a new generation of vectors containing promoters for specific bacteriophage RNA polymerases, it is possible to synthesize high-specific-activity antisense RNA probes. If a probe is synthesized that is complementary to an intron–exon–intron junction, it is possible to detect the primary transcript that will protect the full-length probe. The in situ hybridization assay gives excellent single-cell anatomical resolution but fails to resolve the different RNA species." @default.
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- W1673295677 date "1989-01-01" @default.
- W1673295677 modified "2023-10-14" @default.
- W1673295677 title "Quantitation of Nuclear Low-Level Gene Expression in Central Nervous System Using Solution Hybridization and in Situ Hybridization" @default.
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- W1673295677 doi "https://doi.org/10.1016/b978-0-12-185251-1.50021-0" @default.
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